Chromatin Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Chellappan, Srikumar P. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:2nd ed. 2009.
Colección:Methods in Molecular Biology, 523
Materias:
Tabla de Contenidos:
  • Preparation of Chromatin Assembly Extracts from Preblastoderm Drosophila Embryos
  • Analysis of Reconstituted Chromatin Using a Solid-Phase Approach
  • In Vivo Chromatin Decondensation Assays: Molecular Genetic Analysis of Chromatin Unfolding Characteristics of Selected Proteins
  • DNA Methyltransferase Probing of Chromatin Structure Within Populations and on Single Molecules
  • Visualization of the Expression of HMGN Nucleosomal Binding Proteins in the Developing Mouse Embryo and in Adult Mouse Tissues
  • Drug-Induced Premature Chromosome Condensation (PCC) Protocols: Cytogenetic Approaches in Mitotic Chromosome and Interphase Chromatin
  • Analysis of DNA Topology in Yeast Chromatin
  • Preparation and Analysis of Uniquely Positioned Mononucleosomes
  • Monitoring DNA Breaks in Optically Highlighted Chromatin in Living Cells by Laser Scanning Confocal Microscopy
  • Methods to Study Transcription-Coupled Repair in Chromatin
  • Cytometric Analysis of DNA Damage: Phosphorylation of Histone H2AX as a Marker of DNA Double-Strand Breaks (DSBs)
  • Detection of DNA Damage Induced by Topoisomerase II Inhibitors, Gamma Radiation and Crosslinking Agents Using the Comet Assay
  • Analysis of Genomic Aberrations Using Comparative Genomic Hybridization of Metaphase Chromosomes
  • In Vitro Replication Assay with Mammalian Cell Extracts
  • Investigation of Genomic Methylation Status Using Methylation-Specific and Bisulfite Sequencing Polymerase Chain Reaction
  • Biochemical Analysis of Arginine Methylation in Transcription
  • In Vitro Histone Demethylase Assays
  • Histone Chaperone as Coactivator of Chromatin Transcription: Role of Acetylation
  • Histone Deacetylase Activity Assay
  • In Vitro and In Vivo Assays for Studying Histone Ubiquitination and Deubiquitination
  • Detection of Histone H3 Phosphorylation in Cultured Cells and Tissue Sections by Immunostaining
  • Chromatin Immunoprecipitation Assays: Analyzing Transcription Factor Binding and Histone Modifications In Vivo
  • ChIP on Chip Assays: Genome-Wide Analysis of Transcription Factor Binding and Histone Modifications
  • ChIP-on-Chip Analysis Methods for Affymetrix Tiling Arrays
  • Non-Radioactive Assay Methods for the Assessment of Telomerase Activity and Telomere Length
  • Detecting ATM-Dependent Chromatin Modification in DNA Damage and Heat Shock Response.