Bone Research Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Helfrich, Miep H. (Editor), Ralston, Stuart H. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2012.
Edition:2nd ed. 2012.
Series:Methods in Molecular Biology, 816
Subjects:
Table of Contents:
  • Primary Human Osteoblast Cultures
  • Osteoblast Isolation from Murine Calvaria and Long Bones
  • Rat Osteoblast Cultures
  • Isolation of primary avian Osteocytes
  • Isolation of Mouse Osteocytes using Cell Fractionation for Gene Expression Analysis
  • Studying Osteocyte Function using the Cell Lines MLO-Y4 and MLO-A5
  • Isolation, Differentiation and Characterisation of Human Bone Marrow Stem Cells in vitro and in vivo
  • Rodent Osteoclast Cultures
  • Isolation and Culture of Primary Chicken Osteoclasts
  • ISOLATION AND PURIFICATION OF RABBIT OSTEOCLASTS
  • Generation of Human Osteoclasts from Peripheral Blood
  • Osteoclast Formation in Mouse Co-Cultures
  • Rankl- Mediated Osteoclast Formation from Murine RAW 264.7 Cells
  • Transfection of Osteoclasts and Osteoclast Precursors
  • Analysis of Signalling Pathways by Western Blotting and Immunoprecipitation
  • Analysis of Transcriptional Regulation in Bone Cells
  • Extraction of Nucleic Acids from Bone
  • Analysis of Gene Expression in Bone by Quantitative RT/PCR
  • Histomorphometry in Rodents
  • Studying Gene Expression in Bone by in situ Hybridization
  • Immunostaining of Skeletal Tissue
  • Techniques for the Study of Apoptosis in Bone
  • Transmission Electron Microscopy of Bone
  • Scanning Electron Microscopy of Bone
  • Fluorescence Imaging of Osteoclasts using Confocal Microscopy
  • Live Imaging of Bone Cell and Organ Cultures
  • Analysis of Bone Architecture in Rodents using Micro Computed Tomography
  • Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents
  • Quantitative X-ray Imaging of Rodent Bone by Faxitron
  • Bioluminescence Imaging of Bone Metastasis in Rodent
  • Fourier Transform Infrared Imaging of Bone
  • Raman Microscopy of Bone
  • The Calvarial Injection Assay
  • Ovariectomy/Orchidectomy in Rodents
  • Mechanical Properties of Bone ex vivo
  • Mechanical Stimulation of Bone Cells using Fluid Flow
  • using CELL and organ culture models TO analyse responses OF bone cellS to mechanical STIMULATION .In vivo Mechanical Loading.