Auditory and Vestibular Research : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2009.
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| Edición: | 1st ed. 2009. |
| Colección: | Methods in Molecular Biology,
493 |
| Materias: |
Tabla de Contenidos:
- Nucleic Acid Protocols
- RNA Isolation from Xenopus Inner Ear Sensory Endorgans for Transcriptional Profiling and Molecular Cloning
- Synthesis of Biotin-Labeled RNA for Gene Expression Measurements Using Oligonucleotide Arrays
- In situ Hybridization Approach to Study mRNA Expression and Distribution in Cochlear Frozen Sections
- Lineage Analysis of Inner Ear Cells Using Genomic Tags for Clonal Identification
- Genetic Fate-Mapping Approaches: New Means to Explore the Embryonic Origins of the Cochlear Nucleus
- The Practical Use of Cre and loxP Technologies in Mouse Auditory Research
- Helios® Gene Gun–Mediated Transfection of the Inner Ear Sensory Epithelium
- Electroporation-Mediated Gene Transfer to the Developing Mouse Inner Ear
- Isolation of Sphere-Forming Stem Cells from the Mouse Inner Ear
- Molecular Biology of Vestibular Schwannomas
- Multilocus Sequence Typing and Pulsed Field Gel Electrophoresis of Otitis Media Causing Pathogens
- Fluorescence “In Situ” Hybridization for the Detection of Biofilm in the Middle Ear and Upper Respiratory Tract Mucosa
- Positional Cloning of Deafness Genes
- Amino Acid Protocols
- Twist-Off Purification of Hair Bundles
- Yeast Two-Hybrid Screening to Test for Protein–Protein Interactions in the Auditory System
- The Use of 2-D Gels to Identify Novel Protein–Protein Interactions in the Cochlea
- Identification of Functionally Important Residues/Domains in Membrane Proteins Using an Evolutionary Approach Coupled with Systematic Mutational Analysis
- In Vivo Verification of Protein Interactions in the Inner Ear by Coimmunoprecipitation
- Identification of Transcription Factor–DNA Interactions Using Chromatin Immunoprecipitation Assays
- Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Proteins in Inner-Ear Sensory Epithelia
- Multiplexed Isobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches to Auditory Research
- Imaging Protocols
- Fluorescence Microscopy Methods in the Study of Protein Structure and Function
- Ion Imaging in the Cochlear Hair Cells
- Atomic Force Microscopy in Studies of the Cochlea.