Cereal Genomics : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2014.
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| Edición: | 1st ed. 2014. |
| Colección: | Methods in Molecular Biology,
1099 |
| Materias: |
Tabla de Contenidos:
- DNA Extraction from Vegetative Tissue for Next Generation Sequencing
- DNA Extraction from Rice Endosperm (Including a Protocol for Extraction of DNA from Ancient Seed Samples)
- RNA Extraction from Cereal Vegetative Tissue
- RNA Extraction from Developing or Mature Wheat Seeds
- cDNA Library Preparation
- Preparation of High Molecular Weight gDNA and Bacterial Artificial Chromosome (BAC) Libraries in Plants
- The Polymerase Chain Reaction (PCR) - General Methods
- Mutation and Mutation Screening
- The Quantitative Real-Time Polymerase Chain Reaction for the Analysis of Plant Gene Expression
- Cloning of DNA Fragments: Ligation Reactions in Agarose Gel
- Rapid Cloning of Genes and Promoters for Functional Analyses
- Genome Walking
- Functional Analysis by Protein Biochemistry
- Genomic Southern Blot analysis
- Northern Hybridization: A Proficient Method for Detection of Small RNAs and MicroRNAs
- Protein Blotting-Protocol for Beginners
- Genetic Transformation of Wheat via Particle Bombardment
- Sorghum Genetic Transformation by Particle Bombardment
- Genetic Transformation of Wheat via Agrobacterium-mediated DNA Delivery
- A Protocol for High-throughput Agrobacterium-mediated Barley Transformation
- Agrobacterium-mediated Transformation- Rice Transformation
- Agrobacterium-mediated Transformation of Maize (Zea mays) Immature Embryos
- A Technical Platform for PCR-based SNP Screening in Cereals and Other Crops
- A Method for Discovery of Genome-wide SNP Between Any Two Genotypes from Whole Genome Re-sequencing Data.