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171013s2018 xxu| s |||| 0|eng d |
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|a 9781493973392
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| 024 |
7 |
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|a 10.1007/978-1-4939-7339-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Argonaute Proteins :
|b Methods and Protocols /
|c edited by Katsutomo Okamura, Kotaro Nakanishi.
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| 250 |
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|a 1st ed. 2018.
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| 260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2018.
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| 300 |
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|a XIII, 260 p. 55 illus., 44 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1680
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| 505 |
0 |
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|a Cloning and Identification of Recombinant-Argonaute Bound Small RNAs using Next-Generation Sequencing -- Quantification of miRNAs Co-Immunoprecipitated with Argonaute Proteins using SYBR Green-Based qRT-PCR -- Gateway to Understanding Argonaute Loading of Single-Stranded RNAs: Preparation of Deep Sequencing Libraries with In Vitro Loading Samples -- Dumbbell-PCR for Discriminative Quantification of a Small RNA Variant -- MicroRNA Detection by Whole-Mount In Situ Hybridization in C. elegans -- cCLIP-Seq: Retrieval of Chimaeric Reads from HITS-CLIP (CLIP-Seq) Libraries -- Kinetic Analysis of Small Silencing RNA Production by Human and Drosophila Dicer Enzymes In Vitro -- Nucleic Acid-Binding Assay of Argonaute Protein using Fluorescence Polarization -- Reconstitution of RNA Interference Machinery -- Single-Molecule Analysis for RISC Assembly and Target Cleavage -- Profiling Open Chromatin Structure in the Ovarian Somatic Cells using ATAC-Seq -- Assessing miR-451 Activity and Its Role in Erythropoiesis -- Functional Analysis of MicroRNAs in Neurogenesis during Mouse Cortical Development -- Cellular Approaches in Investigating Argonaute2-Dependent RNA Silencing -- Genomic Tagging of AG01 using CRISPR/Cas9-Mediated Homologous Recombination -- Accurate Profiling and Quantification of tRNA Fragments from RNA-Seq Data: A vade mecum for MINTmap.
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| 650 |
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|a Proteins .
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| 650 |
1 |
4 |
|a Protein Science.
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| 700 |
1 |
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|a Okamura, Katsutomo.
|e editor.
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| 700 |
1 |
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|a Nakanishi, Kotaro.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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