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Argonaute Proteins : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Okamura, Katsutomo. (Editor), Nakanishi, Kotaro. (Editor)
Format: eBook
Language:English
Published: New York, NY : Springer New York : Imprint: Humana, 2018.
Edition:1st ed. 2018.
Series:Methods in Molecular Biology, 1680
Subjects:
Table of Contents:
  • Cloning and Identification of Recombinant-Argonaute Bound Small RNAs using Next-Generation Sequencing
  • Quantification of miRNAs Co-Immunoprecipitated with Argonaute Proteins using SYBR Green-Based qRT-PCR
  • Gateway to Understanding Argonaute Loading of Single-Stranded RNAs: Preparation of Deep Sequencing Libraries with In Vitro Loading Samples
  • Dumbbell-PCR for Discriminative Quantification of a Small RNA Variant
  • MicroRNA Detection by Whole-Mount In Situ Hybridization in C. elegans
  • cCLIP-Seq: Retrieval of Chimaeric Reads from HITS-CLIP (CLIP-Seq) Libraries
  • Kinetic Analysis of Small Silencing RNA Production by Human and Drosophila Dicer Enzymes In Vitro
  • Nucleic Acid-Binding Assay of Argonaute Protein using Fluorescence Polarization
  • Reconstitution of RNA Interference Machinery
  • Single-Molecule Analysis for RISC Assembly and Target Cleavage
  • Profiling Open Chromatin Structure in the Ovarian Somatic Cells using ATAC-Seq
  • Assessing miR-451 Activity and Its Role in Erythropoiesis
  • Functional Analysis of MicroRNAs in Neurogenesis during Mouse Cortical Development
  • Cellular Approaches in Investigating Argonaute2-Dependent RNA Silencing
  • Genomic Tagging of AG01 using CRISPR/Cas9-Mediated Homologous Recombination
  • Accurate Profiling and Quantification of tRNA Fragments from RNA-Seq Data: A vade mecum for MINTmap.