Argonaute Proteins : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2018.
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| Edición: | 1st ed. 2018. |
| Colección: | Methods in Molecular Biology,
1680 |
| Materias: |
Tabla de Contenidos:
- Cloning and Identification of Recombinant-Argonaute Bound Small RNAs using Next-Generation Sequencing
- Quantification of miRNAs Co-Immunoprecipitated with Argonaute Proteins using SYBR Green-Based qRT-PCR
- Gateway to Understanding Argonaute Loading of Single-Stranded RNAs: Preparation of Deep Sequencing Libraries with In Vitro Loading Samples
- Dumbbell-PCR for Discriminative Quantification of a Small RNA Variant
- MicroRNA Detection by Whole-Mount In Situ Hybridization in C. elegans
- cCLIP-Seq: Retrieval of Chimaeric Reads from HITS-CLIP (CLIP-Seq) Libraries
- Kinetic Analysis of Small Silencing RNA Production by Human and Drosophila Dicer Enzymes In Vitro
- Nucleic Acid-Binding Assay of Argonaute Protein using Fluorescence Polarization
- Reconstitution of RNA Interference Machinery
- Single-Molecule Analysis for RISC Assembly and Target Cleavage
- Profiling Open Chromatin Structure in the Ovarian Somatic Cells using ATAC-Seq
- Assessing miR-451 Activity and Its Role in Erythropoiesis
- Functional Analysis of MicroRNAs in Neurogenesis during Mouse Cortical Development
- Cellular Approaches in Investigating Argonaute2-Dependent RNA Silencing
- Genomic Tagging of AG01 using CRISPR/Cas9-Mediated Homologous Recombination
- Accurate Profiling and Quantification of tRNA Fragments from RNA-Seq Data: A vade mecum for MINTmap.