Checkpoint Controls and Cancer : Volume 2: Activation and Regulation Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Schönthal, Axel H. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2004.
Edición:1st ed. 2004.
Colección:Methods in Molecular Biology, 281
Materias:
Tabla de Contenidos:
  • Protocols for the Study of Checkpoint Regulatory Components
  • Analysis of RB Action in DNA Damage Checkpoint Response
  • Interaction Between the Retinoblastoma Protein and Protein Phosphatase 1 During the Cell Cycle
  • Generation of p53 Target Database Via Integration of Microarray and Global p53 DNA-Binding Site Analysis
  • Functional Analysis of CDK Inhibitor p21WAF1
  • Analysis of p21CDKN1A Recruitment to DNA Excision Repair Foci in the UV-Induced DNA Damage Response
  • Quantitative Determination of p16 Gene Expression by RT-PCR
  • Measuring Cyclin-Dependent Kinase Activity
  • Determination of the Catalytic Activities of mTOR and Other Members of the Phosphoinositide-3-Kinase-Related Kinase Family
  • CHK1 Kinase Activity Assay
  • Assaying Cdc25 Phosphatase Activity
  • Analyzing the Regulation and Function of ATM
  • Use of siRNA to Study the Function of MDC1 in DNA Damage Responses
  • Functional Analysis of APC-Cdh1
  • Purification of the Mitotic Checkpoint Complex, an Inhibitor of the APC/C From HeLa Cells
  • Analysis of the Spindle-Assembly Checkpoint in HeLa Cells
  • Functional Analysis of the Spindle-Checkpoint Proteins Using an In Vitro Ubiquitination Assay
  • Studying Consequences of Checkpoint Pathway Activation
  • Analysis of Checkpoint Responses to Histone Deacetylase Inhibitors
  • Biochemical Analysis of the Cell Cycle and Cell Cycle Checkpoints in Transiently Transfected Cells After Collection With Magnetic Beads
  • Analysis of DNA Repair and Chromatin Assembly In Vitro Using Immobilized Damaged DNA Substrates
  • Analyzing Cell Cycle Checkpoints After Ionizing Radiation
  • FACS-Based Detection of Phosphorylated Histone H3 for the Quantitation of Mitotic Cells
  • Analysis of Cell Cycle by Flow Cytometry
  • Analyzing Markers of Apoptosis In Vitro
  • Analysis of Telomerase Activity and Telomere Function in Cancer.