Chloroplast Research in Arabidopsis : Methods and Protocols, Volume I /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Jarvis, R. Paul. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2011.
Edition:1st ed. 2011.
Series:Methods in Molecular Biology, 774
Subjects:
Table of Contents:
  • Screening or Selection for Chloroplast Biogenesis Mutants of Arabidopsis, Following Chemical or Insertional Mutagenesis
  • Analysis of Plastid Number, Size, and Distribution in Arabidopsis Plants by Light and Fluorescence Microscopy
  • Immunofluorescence Microscopy for Localization of Arabidopsis Chloroplast Proteins
  • Transient Expression and Analysis of Chloroplast Proteins in Arabidopsis Protoplasts
  • Visualisation of Stromules on Arabidopsis Plastids
  • Analysis of Chloroplast Movement and Relocation in Arabidopsis
  • Studying Starch Content and Sedimentation of Amyloplast Statoliths In Arabidopsis Roots
  • Studying Arabidopsis Chloroplast Structural Organization Using Transmission Electron Microscopy
  • Transplastomics in Arabidopsis: Progress Towards Developing an Efficient Method
  • Isolation, Quantification, and Analysis of Chloroplast DNA
  • Measurement of Transcription Rates in Arabidopsis Chloroplasts
  • Studying the Structure and Processing of Chloroplast Transcripts
  • In Vitro RNA Binding Assay for Studying Trans-Factors for RNA Editing in Chloroplasts
  • Studying Translation in Arabidopsis Chloroplasts
  • Studying Proteases and Protein Turnover in Arabidopsis Chloroplasts
  • In Silico Methods for the Identification of Organellar and Suborganellar Targeting Peptides in Arabidopsis Chloroplast Proteins and for Predicting the Topology of Membrane Proteins
  • Rapid Isolation of Arabidopsis Chloroplasts and Their Use for In Vitro Protein Import Assays
  • Energetic Manipulation of Chloroplast Protein Import and the Use of Chemical Cross-Linkers to Map Protein-Protein Interactions
  • Isolation of Arabidopsis Thylakoid Membranes and Their Use for In Vitro Protein Insertion or Transport Assays
  • Determining the Location of an Arabidopsis Chloroplast Protein Using In Vitro Import Followed by Fractionation and Alkaline Extraction
  • Studying Arabidopsis Envelope Protein Localization and Topology Using Thermolysin and Trypsin Proteases.