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100301s2004 xxu| s |||| 0|eng d |
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|a 9781592594061
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| 024 |
7 |
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|a 10.1385/1592594069
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Cancer Cell Culture :
|b Methods and Protocols /
|c edited by Simon P. Langdon.
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| 250 |
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|a 1st ed. 2004.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2004.
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| 300 |
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|a XII, 360 p. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Medicine,
|v 88
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| 505 |
0 |
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|a to Cancer Cell Culture -- Basic Principles of Cancer Cell Culture -- Essential Techniques of Cancer Cell Culture -- Characterization and Authentication -- Characterization and Authentication of Cancer Cell Lines -- Authentication of Cancer Cell Lines by DNA Fingerprinting -- Cytogenetic Characterization of Tumor Cell Lines -- Isolation and Culture of Specialized Cell Types -- Isolation and Culture of Colon Cancer Cell Lines -- Isolation and Culture of Melanoma Cell Lines -- Isolation and Culture of Human Brain Tumor Cells -- Isolation and Culture of Renal Cancer Cell Lines -- Isolation and Culture of Prostate Cancer Cell Lines -- Isolation and Culture of Ovarian Cancer Cell Lines -- Isolation and Culture of Leukemia Cell Lines -- Functional Assays in Cell Culture -- Cell Sensitivity Assays: Clonogenic Assay -- Cell Sensitivity Assays: The MTT Assay -- PARP Cleavage as a Means of Assessing Apoptosis -- Detection of Apoptosis by the TUNEL Assay -- Apoptosis Measurement by Annexin V Staining -- Cell Adhesion Assays -- Radial Monolayer Cell Migration Assay -- Invasion and Motility Assays -- Identification of Senescence in Cancer Cells -- Angiogenesis Assays -- Flow Cytometric DNA Analysis of Human Cancer Cell Lines -- Modification of Cancer Cells -- DNA-Mediated Gene Transfer -- Development of Drug-Resistant Models -- Immortalization of Human Prostate Cells With the Human Papillomavirus Type 16 E6 Gene -- The Use of Matrigel to Facilitate the Establishment of Human Cancer Cell Lines as Xenografts -- Coculture Systems -- Coculture of Prostate Cancer Cells -- Coculture of Ovarian Cells Using Porous Tissue Culture Inserts -- Cell Line Contamination -- Cell Culture Contamination -- Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction -- Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics.
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| 650 |
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0 |
|a Oncology .
|
| 650 |
1 |
4 |
|a Oncology.
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| 700 |
1 |
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|a Langdon, Simon P.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|