Bacterial Protein Secretion Systems : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2017.
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| Edición: | 1st ed. 2017. |
| Colección: | Methods in Molecular Biology,
1615 |
| Materias: |
Tabla de Contenidos:
- Identification of Protein Secretion Systems in Bacterial Genomes using MacSyFinder
- Protein Sorting Prediction
- Cell Fractionation
- Defining Lipoprotein Localisation by Fluorescence Microscopy
- Components Sub-cellular Localization: Identification of Lipoproteins using Globomycin and Radioactive Palmitate
- Defining Membrane Protein Localization by Isopycnic Density Gradients
- Components Sub-cellular Localisation: Cell Surface Exposure
- Probing Inner Membrane Protein Topology by Proteolysis
- Mapping of Membrane Protein Topology by Substituted Cysteine Accessibility Method (SCAMTM)
- Defining Membrane Protein Topology using pho-lac Reporter Fusions
- In vivo and in vitro Protein-peptidoglycan Interactions
- Measure of Peptidoglycan Hydrolase Activity
- Protein-protein Interaction: Bacterial Two-Hybrid
- Protein-protein Interactions: Yeast two-hybrid
- Protein-protein Interactions: Cytology Two-hybrid
- Fusion Reporter Approaches to Monitor Transmembrane Helix Interactions in Bacterial Membranes
- Protein-protein Interactions: Co-immunoprecipitation
- Protein-protein Interaction: Tandem Affinity Purification in Bacteria
- Site-directed and Time-resolved Photocrosslinking in Cells Metabolically Labeled With Radioisotopes
- Protein-protein Interactions: Pull-down Assays
- Protein-protein interactions: Surface Plasmon Resonance
- Assessing Energy-dependent Protein Conformational Changes in the TonB System
- Defining Assembly Pathways by Fluorescence Microscopy
- Large Complexes: Cloning Strategy, Production, and Purification
- Shearing and Enrichment of Extracellular Type IV Pili
- Blue Native PAGE Analysis of Bacterial Secretion Complexes
- In situ Imaging of Bacterial Secretion Systems by Electron Cryo-tomography
- Structural Analysis of Protein Complexes by Cryo Electron Microscopy
- Bacterial Filamentous Appendages Investigated by Solid-state NMR Spectroscopy
- Identification of Effectors: Precipitation of Supernatant Material
- Energy Requirements for Protein Secretion via the Flagellar type-III Secretion System
- Screening for Secretion of the Type VI Secretion Protein Hcp by Enzyme-Linked Immunosorbent Assay and Colony Blot
- Effector Translocation: Cya Reporter Assay
- Monitoring Effector Translocation with the TEM-1 Beta-lactamase Reporter System
- Effector Translocation Assay: Differential Solubilization
- Quantitative Determination of Anti-bacterial Activity during Bacterial Co-culture. .