Chaperonin Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2000.
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| Edición: | 1st ed. 2000. |
| Colección: | Methods in Molecular Biology,
140 |
| Materias: |
Tabla de Contenidos:
- Purification of Archaeal Chaperonin from Sulfolobus shibatae
- Purification of Hsp60 from Thermus thermophilus
- Purification of GroEL from an Overproducing E. coliStrain
- Purification of GroES from an Overproducing E. coliStrain
- Purification of the Gp31 Co-chaperonin of BacteriophageT4
- Removing Trace Fluorescent Contaminants from GroEL Preparations
- Assembly and Disassembly of GroEL and GroES Complexes
- GroEL/GroES Interaction Assayed by Protease Protection
- Determination of Chaperonin Activity In Vivo
- Interaction of Nonnative Polypeptide Substrates with the Escherichia coli Chaperonin GroEL
- Prevention of Rhodanese Aggregation by the Chaperonin GroEL
- Refolding of Bovine Mitochondrial Rhodanese by Chaperonins GroEL and GroES
- Assay of Malate Dehydrogenase
- Assay of Chaperonin-Assisted Refolding of Citrate Synthase
- Purification of Yeast Mitochondrial Hsp60
- Preparation of Recombinant Human Hsp10
- Purification of the Cytosolic ChaperoninTRiC from Bovine Testis
- Monitoring Actin Folding
- Folding Assays
- Purification of Prefoldin
- Purification of GimC from Saccharomyces cerevisiae
- Analysis of Eukaryotic Molecular Chaperone Complexes Involved in Actin Folding.