Bacterial Chemosensing : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
New York, NY :
Springer New York : Imprint: Humana,
2018.
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| Edition: | 1st ed. 2018. |
| Series: | Methods in Molecular Biology,
1729 |
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Table of Contents:
- The Diversity of Bacterial Chemosensing
- Transmembrane Signal Transduction in Bacterial Chemosensing
- Two Spatial Chemotaxis Assays: The Nutrient-Depleted Chemotaxis Assay and the Agarose-Plug-Bridge Assay
- Quantification of Bacterial Chemotaxis Responses at the Mouths of Hydrogel Capillaries
- A Static Microfluidic Device for Investigating the Chemotaxis Response to Stable, Non-Linear Gradients
- Visualizing Chemoattraction of Planktonic Cells to a Biofilm
- Labeling Bacterial Flagella with Fluorescent Dyes
- All-Codon Mutagenesis for Structure-Function Studies of Chemotaxis Signaling Proteins
- Mutational Analysis of Binding Protein/Chemoreceptor Interactions
- In Vitro Assay for Measuring Receptor-Kinase Activity in the Bacillus subtilis Chemotaxis Pathway
- FRET Analysis of the Chemotaxis Pathway Response
- Monitoring Two-Component Sensor Kinases with a Chemotaxis Signal Readout
- Analyzing Protein Domain Interactions in Chemoreceptors by In Vivo PEGylation
- Tuning Chemoreceptor Signaling by Positioning Aromatic Residues at the Lipid-Aqueous Interface
- Analyzing Chemoreceptor Interactions In Vivo with the Tri-Functional Crosslinker TMEA
- Use of Cryo-EM to Study the Structure of Chemoreceptor Arrays In Vivo
- Visualizing Chemoreceptor Arrays in Bacterial Minicells by Cryo-Electron Tomography and Subtomogram Analysis
- Bacterial Chemoreceptor Imaging at High Spatio-Temporal Resolution Using Photoconvertible Fluorescent Proteins
- Imaging of Single Dye-Labeled Chemotaxis Proteins in Live Bacteria Using Electroporation
- Fluorescence Anisotropy to Detect In Vivo Stimulus-Induced Changes in Chemoreceptor Packing
- Chemotaxis to Atypical Chemoattractants by Soil Bacteria
- Screening Chemoreceptor-Ligand Interactions by High-Throughput Thermal-Shift Assays
- High-Throughput Screening to Identify Chemoreceptor Ligands.- Identification of Specific Ligands for Sensory Receptors by Small-Molecule Ligand Arrays and Surface Plasmon Resonance.- Fluorescence Measurement of Kinetics of CheY Autophosphorylation with Small Molecule Phosphodonors
- Synthesis of a Stable Analog of the Phosphorylated Form of CheY: Phosphono-CheY
- Quantitative Modeling of Flagellar Motor-Mediated Adaptation
- Molecular Modeling of Chemoreceptor:Ligand Interactions
- Phylogenetic and Protein Sequence Analysis of Bacterial Chemoreceptors.