Cyclooxygenases : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Ayoub, Samir S. (Editor ), Flower, Roderick J. (Editor ), Seed, Michael. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2010.
Edición:1st ed. 2010.
Colección:Methods in Molecular Biology, 644
Materias:
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020 |a 9781597453646 
024 7 |a 10.1007/978-1-59745-364-6  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a Cyclooxygenases :  |b Methods and Protocols /  |c edited by Samir S. Ayoub, Roderick J. Flower, Michael Seed. 
250 |a 1st ed. 2010. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2010. 
300 |a X, 218 p. 38 illus. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 644 
505 0 |a In Vitro Protocols for Studying Expression and Activity of Cyclooxygenase Enzymes and Characterisation of Their Inhibition Patterns -- In Vitro Cyclo-oxygenase Expression and Activity Protocols: Introduction to Part I -- Techniques Used to Study Regulation of Cyclooxygenase-2 Promoter Sites -- Purification of Recombinant Human COX-1 and COX-2 -- Cloning and Expression of Cyclooxygenase-1 and Cyclooxygenase-2 -- Expression of Cyclooxygenase Isoforms in the Baculovirus Expression System -- Peroxidase Active Site Activity Assay -- Study of Inhibitors of the PGH Synthases for which Potency Is Regulated by the Redox State of the Enzymes -- Different Methods for Testing Potential Cyclooxygenase-1 and Cyclooxygenase-2 Inhibitors -- In Vitro Cyclooxygenase Activity Assay in Tissue Homogenates -- Techniques Used to Characterize the Binding of Cyclooxygenase Inhibitors to the Cyclooxygenase Active Site -- In Vitro Assays for Cyclooxygenase Activity and Inhibitor Characterization -- Extraction and Measurement of Prostanoids and Isoprostanes -- Extraction and Measurement of Prostanoids and Isoprostanes: Introduction to Part II -- Prostanoid Extraction and Measurement -- Measurement of 8-Iso-Prostaglandin F2? in Biological Fluids as a Measure of Lipid Peroxidation -- In Vivo Models to Study Involvement of Cyclooxygenase Products in Health and Disease -- In Vivo Models to Study Cyclooxygenase Products in Health and Disease: Introduction to Part III -- Protocols to Assess the Gastrointestinal Side Effects Resulting from Inhibition of Cyclo-Oxygenase Isoforms -- Cyclooxygenase Enzymes and Their Products in the Carrageenan-Induced Pleurisy in Rats -- Iloprost-Induced Nociception: Determination of the Site of Anti-nociceptive Action of Cyclooxygenase Inhibitors and the Involvement of Cyclooxygenase Products in Central Mechanisms of Nociception. 
650 0 |a Pharmacology. 
650 0 |a Biochemical engineering. 
650 1 4 |a Pharmacology/Toxicology. 
650 2 4 |a Biochemical Engineering. 
700 1 |a Ayoub, Samir S.  |e editor. 
700 1 |a Flower, Roderick J.  |e editor. 
700 1 |a Seed, Michael.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks