G Protein-Coupled Receptors in Drug Discovery : Methods and Protocols /
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Humana,
2015.
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| Edición: | 2nd ed. 2015. |
| Colección: | Methods in Molecular Biology,
1335 |
| Materias: |
Tabla de Contenidos:
- Purification of Stabilized GPCRs for Structural and Biophysical Analyses
- Purification and Crystallization of a Thermostabilized Agonist-Bound Conformation of the Human Adenosine A2A Receptor
- 2D Projection Analysis of GPCR Complexes by Negative Stain Electron Microscopy
- Nuts and Bolts of CF3 and CH3 NMR Towards the Understanding of Conformational Exchange of GPCRs
- Single-Molecule Fluorescence Microscopy for the Analysis of Fast Receptor Dynamics
- Quantitative Multi-Color Detection Strategies for Bioorthogonally-Labeled GPCRs
- Approaches to Characterize and Quantify Oligomerization of GPCRs
- Monitoring G Protein Activation in Cells with BRET
- Use of Fluorescence Indicators in Receptor Ligands
- Detection and Quantification of Intracellular Signalling Using FRET-Based Biosensors and High Content Imaging
- The Measurement of Receptor Signaling Bias
- Approaches to Assess Functional Selectivity in GPCRs: Evaluating G Protein Signaling in an Endogenous Environment
- Bioluminescence Resonance Energy Transfer Approaches to Discover Bias in GPCR Signaling
- Virus-Mediated Expression of DREADDs for In Vivo Metabolic Studies
- High-Throughput Screening for Allosteric Modulators of GPCRs
- Radioligand Binding Assay for an Exon 11-Associated Mu Opioid Receptor Target
- Docking and Virtual Screening Strategies for GPCR Drug Discovery
- The Dynamic Process of Drug-GPCR Binding at either Orthosteric or Allosteric Sites Evaluated by Metadynamics
- Experiment-Guided Molecular Modeling of Protein-Protein Complexes Involving GPCRs
- Interaction Fingerprints and Their Applications to Identify Hot Spots.