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02779nam a22003015i 4500 |
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20210428132857.0 |
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cr nn 008mamaa |
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130321s2013 xxu| s |||| 0|eng d |
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|a 9781627033022
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| 024 |
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|a 10.1007/978-1-62703-302-2
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
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|a Dictyostelium discoideum Protocols /
|c edited by Ludwig Eichinger, Francisco Rivero.
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| 250 |
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|a 2nd ed. 2013.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2013.
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| 300 |
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|a XII, 479 p. 110 illus., 55 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
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|a Methods in Molecular Biology,
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| 505 |
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|a The Amoebozoa -- The Model Organism Dictyostelium discoideum.-Comparative Genomics of the Dictyostelids -- One Stop Shop for Everything Dictyostelium: DictyBase and the Dicty Stock Center in 2012 -- Fluorescent Reporters and Methods to Analyze Fluorescent Signals.-Collection and Cultivation of Dictyostelids from the Wild.-Identification and Verification of microRNAs by High-throughput Sequencing -- Transcriptional Profiling of Dictyostelium with RNA Sequencing -- Analysis of Chromatin Organization by Deep Sequencing Technologies -- Pharmacogenetics of Resistance to Cisplatin and Other Anti-cancer Drugs and the Role of Sphingolipid Metabolism.-N-glycomic and -Glycoproteomic Studies in the Social Amoebae.-Measuring Cheating, Fitness, and Segregation in D. discoideum.-The Application of the Cre-loxP System for Generating Multiple Knock-Out and Knock-In Targeted Loci -- Extrachromosomal Inducible Expression -- Isolation of Dictyostelium Nuclei for Light and Electron Microscopy -- Investigation of DNA Repair Pathway Activity -- Transcript Localization in D. discoideum Cells by RNA FISH -- Analysis of Mitochondrial Gene Expression -- Mitochondrial Respiratory Complex Function and the Phenotypic Consequences of Dysfunction -- Micropipette Aspiration for Studying Cellular Mechanosensory Responses and Mechanics -- Quantitative Analysis of Phagocytosis and Phagosome Maturation -- Setting Up and Monitoring an Infection of Dictyostelium discoideum with Mycobacteria -- Isolation of Pathogen-containing Vacuoles -- Immuno-magnetic Purification of Fluorescent Legionella-containing Vacuoles -- Secretory Lysosomes in Dictyostelium: Visualization, Characterization and Dynamics -- Monitoring Autophagy in Dictyostelium. .
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| 650 |
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|a Microbiology.
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| 650 |
1 |
4 |
|a Microbiology.
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| 650 |
2 |
4 |
|a Eukaryotic Microbiology.
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| 700 |
1 |
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|a Eichinger, Ludwig.
|e editor.
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| 700 |
1 |
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|a Rivero, Francisco.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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