Dictyostelium discoideum Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Eichinger, Ludwig. (Editor ), Rivero, Francisco. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2013.
Edición:2nd ed. 2013.
Colección:Methods in Molecular Biology,
Materias:
Tabla de Contenidos:
  • The Amoebozoa
  • The Model Organism Dictyostelium discoideum.-Comparative Genomics of the Dictyostelids
  • One Stop Shop for Everything Dictyostelium: DictyBase and the Dicty Stock Center in 2012
  • Fluorescent Reporters and Methods to Analyze Fluorescent Signals.-Collection and Cultivation of Dictyostelids from the Wild.-Identification and Verification of microRNAs by High-throughput Sequencing
  • Transcriptional Profiling of Dictyostelium with RNA Sequencing
  • Analysis of Chromatin Organization by Deep Sequencing Technologies
  • Pharmacogenetics of Resistance to Cisplatin and Other Anti-cancer Drugs and the Role of Sphingolipid Metabolism.-N-glycomic and -Glycoproteomic Studies in the Social Amoebae.-Measuring Cheating, Fitness, and Segregation in D. discoideum.-The Application of the Cre-loxP System for Generating Multiple Knock-Out and Knock-In Targeted Loci
  • Extrachromosomal Inducible Expression
  • Isolation of Dictyostelium Nuclei for Light and Electron Microscopy
  • Investigation of DNA Repair Pathway Activity
  • Transcript Localization in D. discoideum Cells by RNA FISH
  • Analysis of Mitochondrial Gene Expression
  • Mitochondrial Respiratory Complex Function and the Phenotypic Consequences of Dysfunction
  • Micropipette Aspiration for Studying Cellular Mechanosensory Responses and Mechanics
  • Quantitative Analysis of Phagocytosis and Phagosome Maturation
  • Setting Up and Monitoring an Infection of Dictyostelium discoideum with Mycobacteria
  • Isolation of Pathogen-containing Vacuoles
  • Immuno-magnetic Purification of Fluorescent Legionella-containing Vacuoles
  • Secretory Lysosomes in Dictyostelium: Visualization, Characterization and Dynamics
  •  Monitoring Autophagy in Dictyostelium.      .