Genotype Phenotype Coupling : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Zielonka, Stefan. (Editor ), Krah, Simon. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer US : Imprint: Humana, 2020.
Edición:1st ed. 2020.
Colección:Methods in Molecular Biology, 2070
Materias:
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245 1 0 |a Genotype Phenotype Coupling :  |b Methods and Protocols /  |c edited by Stefan Zielonka, Simon Krah. 
250 |a 1st ed. 2020. 
260 # # |a New York, NY :  |b Springer US :  |b Imprint: Humana,  |c 2020. 
300 |a XII, 445 p. 86 illus., 61 illus. in color. :  |b online resource. 
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490 1 |a Methods in Molecular Biology,  |v 2070 
505 0 |a Screening, Post-SELEX Optimization and Application of DNA Aptamers Specific for Tobramycin -- Affitins: Ribosome Display for Selection of Aho7c-based Affinity Proteins -- cDNA Display: A Stable and Simple Genotype–phenotype Coupling Using a Cell-free Translation System -- cDNA Display of Disulfide-containing Peptide Library and in vitro Evolution -- Rapid Antigen and Antibody-like Molecule Discovery by Staphylococcal Surface Display -- Restriction-free Construction of a Phage-presented Very Short Macrocyclic Peptide Library -- In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties -- Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens -- Assessing Antibody Specificity in Human Serum Using Deep Sequence-coupled Biopanning -- Isolation of Antigen-specific VHH Single Domain Antibodies by Combining Animal Immunization with Yeast Surface Display -- Selection and Characterization of Anti-idiotypic Shark Antibody Domains -- Simplifying the Detection of Surface Presentation Levels in Yeast Surface Display by Intracellular tGFP Expression -- Methods for Construction of Yeast Display Libraries of Four-Domain T-cell Receptors -- Isolation of Tailor-made Antibody Fragments from Yeast-displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting -- Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires -- Rapid Generation of Chicken Immune Libraries for Yeast Surface Display -- Ligand Engineering via Yeast Surface Display and Adherent Cell Panning -- Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces Cerevisiae Using Inefficient Ribosomal Skipping -- Chemical Modification of the Yeast Cell Surface Allows the Switch between Display and Soluble Secretion of Full-length Antibodies -- Advanced Establishment of Stable Recombinant Human Suspension Cell Lines Using Geno-Phenotype Coupling Transposon Vectors -- Mammalian Surface Display Screening of Diverse Cystine-Dense Peptide Libraries for Difficult to Drug Targets -- Engineering Antibodies on the Surface of CHO Cells -- Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies. 
650 0 |a Human genetics. 
650 0 |a Proteins . 
650 1 4 |a Human Genetics. 
650 2 4 |a Protein Science. 
700 1 |a Zielonka, Stefan.  |e editor. 
700 1 |a Krah, Simon.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
900 |a Libro descargado a ALEPH en bloque (proveniente de proveedor)