Genotype Phenotype Coupling : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Zielonka, Stefan. (Editor ), Krah, Simon. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer US : Imprint: Humana, 2020.
Edición:1st ed. 2020.
Colección:Methods in Molecular Biology, 2070
Materias:
Tabla de Contenidos:
  • Screening, Post-SELEX Optimization and Application of DNA Aptamers Specific for Tobramycin
  • Affitins: Ribosome Display for Selection of Aho7c-based Affinity Proteins
  • cDNA Display: A Stable and Simple Genotype–phenotype Coupling Using a Cell-free Translation System
  • cDNA Display of Disulfide-containing Peptide Library and in vitro Evolution
  • Rapid Antigen and Antibody-like Molecule Discovery by Staphylococcal Surface Display
  • Restriction-free Construction of a Phage-presented Very Short Macrocyclic Peptide Library
  • In Vitro Maturation of a Humanized Shark VNAR Domain to Improve Its Biophysical Properties
  • Antibody Phage Display: Antibody Selection in Solution Using Biotinylated Antigens
  • Assessing Antibody Specificity in Human Serum Using Deep Sequence-coupled Biopanning
  • Isolation of Antigen-specific VHH Single Domain Antibodies by Combining Animal Immunization with Yeast Surface Display
  • Selection and Characterization of Anti-idiotypic Shark Antibody Domains
  • Simplifying the Detection of Surface Presentation Levels in Yeast Surface Display by Intracellular tGFP Expression
  • Methods for Construction of Yeast Display Libraries of Four-Domain T-cell Receptors
  • Isolation of Tailor-made Antibody Fragments from Yeast-displayed B-Cell Receptor Repertoires by Multiparameter Fluorescence-Activated Cell Sorting
  • Isolation of Anti-Hapten Antibodies by Fluorescence-Activated Cell Sorting of Yeast-Displayed B-Cell Receptor Gene Repertoires
  • Rapid Generation of Chicken Immune Libraries for Yeast Surface Display
  • Ligand Engineering via Yeast Surface Display and Adherent Cell Panning
  • Simultaneous Soluble Secretion and Surface Display of Proteins in Saccharomyces Cerevisiae Using Inefficient Ribosomal Skipping
  • Chemical Modification of the Yeast Cell Surface Allows the Switch between Display and Soluble Secretion of Full-length Antibodies
  • Advanced Establishment of Stable Recombinant Human Suspension Cell Lines Using Geno-Phenotype Coupling Transposon Vectors
  • Mammalian Surface Display Screening of Diverse Cystine-Dense Peptide Libraries for Difficult to Drug Targets
  • Engineering Antibodies on the Surface of CHO Cells
  • Single B Cell Cloning and Production of Rabbit Monoclonal Antibodies.