Current Laboratory Methods in Neuroscience Research /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
New York, NY :
Springer New York : Imprint: Springer,
2014.
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| Edición: | 1st ed. 2014. |
| Colección: | Springer Protocols Handbooks,
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| Materias: |
Tabla de Contenidos:
- Section 1: Neurohistological techniques
- 1. Brain tissue preparation, sectioning and staining
- 2. Brain stereotactic injections
- 3. Tract tracing at light and electron microscopy
- 4. Stereological analysis of neurological tissues
- Section 2: In vitro preparations
- 5. Preparation and use of rodent hippocampal brain slices
- 6. Single-Cell Neuronal Dissociation For Electrophysiological Studies
- 7. Isolation and Culture of Human Neurons, Microglia and Astrocytes
- 8. Isolation and Culture of Neural Stem/progenitor Cells
- 9. Isolation of mitochondria from brain tissue and cells
- 10. Isolation of synaptosomes from archived brain tissues
- 11. Xenopus oocytes isolation and microinjection
- Section 3: Leukocyte isolation and application in neuroscience
- 12. Centrifugal elutriation for studies of neuroimmunity
- 13. Flow cytometry in neuroscience research
- Section 4: Standard Laboratory Nucleic Acid and Protein Detections
- 14. Western Blotting Technique in Biomedical Research
- 15. Polymerase chain reaction (PCR) and Real-time RT PCR
- Section 5: Nanomedicine
- 16. Nanoformulations
- 17. Neuronanomedicine
- Section 6: Bioimaging
- 18. Confocal imaging of nerve cells
- 19. Magnetic resonance imaging (MRI)
- 20. X-Ray, Positron Emission, and Single Photon Emission Tomographic Bioimaging
- 21. Non-Invasive Neurophysiological Imaging with Magnetoencephalography
- 22. Multiphoton Brain Imaging
- Section 7: Neuroelectrophysiology
- 23. Techniques for Extracellular Recordings
- 24. "Blind" patch-clamp recordings from rodent hippocampal slices
- 25. Whole-cell patch clamp recordings
- Section 8: Immunohistochemistry and autoradiography
- 26. Analysis of Receptor Binding And Quantitative Autoradiography
- 27. Techniques in Immunohistochemistry and immunocytochemistry
- Section 9: Analysis of Gene Expression
- 28. Protein identification by mass spectrometry: Proteomics
- 29. DNA Microarrays as a Tool for Neurosciences Research
- 30. Metabolomics
- 31. Bioinformatic Methods and Resources for Neuroscience Research
- Section 10: Animal Models: Behavior and Pathology
- 32. Motor function in rodent models of neurodegenerative disorders
- 33. Humanized mice
- 34. Animal models for PD and ALS
- 35. Animal models for Alzheimer's Disease
- 36. Animal Models: Behavior and Pathology.