Fluorescent Energy Transfer Nucleic Acid Probes : Designs and Protocols /
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| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2006.
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| Edición: | 1st ed. 2006. |
| Colección: | Methods in Molecular Biology,
335 |
| Materias: |
Tabla de Contenidos:
- Design of Energy Transfer Probes
- Selection of Fluorophore and Quencher Pairs for Fluorescent Nucleic Acid Hybridization Probes
- Choosing Reporter-Quencher Pairs for Efficient Quenching Through Formation of Intramolecular Dimers
- Energy Transfer Probes for DNA and RNA Hybridization Detection and Monitoring
- Detection of DNA Hybridization Using Induced Fluorescence Resonance Energy Transfer
- Detecting RNA/DNA Hybridization Using Double-Labeled Donor Probes With Enhanced Fluorescence Resonance Energy Transfer Signals
- Energy Transfer Probes for DNA Breaks Detection and DNA Cleavage Monitoring
- Oscillating Probe for Dual Detection of 5'PO4 and 5'OH DNA Breaks in Tissue Sections
- Using Molecular Beacons for Sensitive Fluorescence Assays of the Enzymatic Cleavage of Nucleic Acids
- A Continuous Assay for DNA Cleavage Using Molecular Break Lights
- Monitoring of DNA Synthesis and Amplification Using Energy Transfer Probes
- Homogenous Detection of Nucleic Acids Using Self-Quenched Polymerase Chain Reaction Primers Labeled With a Single Fluorophore (LUX™ Primers)
- Use of Self-Quenched, Fluorogenic LUX™ Primers for Gene Expression Profiling
- TaqMan® Reverse Transcriptase-Polymerase Chain Reaction Coupled With Capillary Electrophoresis for Quantification and Identification of bcr-abl Transcript Type
- Quantitative TaqMan® Assay for the Detection and Monitoring of Cytomegalovirus Infection in Organ Transplant Patients
- Real-Time Detection and Quantification of Telomerase Activity Utilizing Energy Transfer Primers
- DNA Sequence Analysis and Mutation Detection Using Fluorescence Energy Transfer
- Invader® Assay for Single-Nucleotide Polymorphism Genotyping and Gene Copy Number Evaluation
- Real-Time Quantitative Polymerase Chain Reaction Analysis of Mitochondrial DNA Point Mutation
- Multiplex Single-Nucleotide Polymorphism Detection by Combinatorial Fluorescence Energy Transfer Tags and Molecular Affinity
- High-Throughput Genotyping With Energy Transfer-Labeled Primers
- Determination of Distance and DNA Folding
- Distance Determination in Protein-DNA Complexes Using Fluorescence Resonance Energy Transfer
- Multi-Fluorophore Fluorescence Resonance Energy Transfer for Probing Nucleic Acids Structure and Folding
- Dna-Based Biosensors Utilizing Energy Transfer
- Fluorescent DNAzyme Biosensors for Metal Ions Based on Catalytic Molecular Beacons
- Fluorescent Energy Transfer Readout of an Aptazyme-Based Biosensor
- Fluorescence Resonance Energy Transfer in the Studies of Guanine Quadruplexes
- Solution-Phase Molecular-Scale Computation With Deoxyribozyme-Based Logic Gates and Fluorescent Readouts.