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02758nam a22002895i 4500 |
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100301s2001 xxu| s |||| 0|eng d |
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|a 9781592590575
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024 |
7 |
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|a 10.1385/1592590578
|2 doi
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040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
1 |
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|a DNA Topoisomerase Protocols :
|b Volume II: Enzymology and Drugs /
|c edited by Neil Osheroff, Mary-Ann Bjornsti.
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250 |
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|a 1st ed. 2001.
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260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2001.
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300 |
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|a XIV, 334 p. :
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 95
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|a Assaying DNA Topoisomerase I Relaxation Activity -- DNA Topoisomerase II-Catalyzed DNA Decatenation -- Plasmid DNA Supercoiling by DNA Gyrase -- Analyzing Reverse Gyrase Activity -- Topoisomerase II-Catalyzed ATP Hydrolysis as Monitored by Thin-Layer Chromatography -- Use of a Real-Time, Coupled Assay to Measure the ATPase Activity of DNA Topoisomerase II -- Analysis of Topoisomerase-DNA Interactions by Electrophoretic Mobility Shift Assay -- Filter Binding Assays for Topoisomerase—DNA Complexes -- DNATopoisomerase I-Mediated Nicking of Circular Duplex DNA -- Analysis of Cleavage Complexes Using Reactive Inhibitor Derivatives -- Uncoupling of Topoisomerase-Mediated DNA Cleavage and Religation -- Synthesis and Use of DNA Containing a 5?-Bridging Phosphorothioate as a Suicide Substrate for Type I DNA Topoisomerases -- Isolation of Covalent Enzyme-DNA Complexes -- ICE Bioassay -- DNA-Unwinding Test Using Eukaryotic DNA Topoisomerase I -- Drug—DNA Interactions -- Quinolone Interactions with DNA and DNA Gyrase -- Bactericidal Assays for Fluoroquinolones -- Drug Toxicity in E. coli Cells Expressing Human Topoisomerase I -- Drug-Induced Cytotoxicity in Tissue Culture -- Analysis of Drug Uptake-Flow Cytometry -- Cell-Cycle Analysis of Drug-Treated Cells -- Assaying Drug-Induced Apoptosis -- Single-Strand Conformational Polymorphism Analysis of DNA Topoisomerases I and II -- PCR-Based Cloning of DNA Topoisomerase Genes -- Topoisomerase II-Catalyzed Relaxation and Catenation of Plasmid DNA -- Topoisomerase II-Mediated Cleavage of Plasmid DNA -- Drug-induced Stabilization of Covalent DNA Topoisomerase I-DNA Intermediates -- Studying DNATopoisomerase I-Targeted Drugs In the Yeast -- Yeast Systems for Demonstrating the Targets of Anti-Topoisomerase II Agents.
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650 |
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|a Pharmacy.
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650 |
1 |
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|a Pharmacy.
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1 |
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|a Osheroff, Neil.
|e editor.
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700 |
1 |
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|a Bjornsti, Mary-Ann.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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773 |
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|t Springer eBooks
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