|
|
|
|
| LEADER |
03948nam a22002895i 4500 |
| 001 |
000281158 |
| 005 |
20220117131542.0 |
| 007 |
cr nn 008mamaa |
| 008 |
100301s2001 xxu| s |||| 0|eng d |
| 020 |
|
|
|a 9781592592272
|
| 024 |
7 |
|
|a 10.1385/1592592279
|2 doi
|
| 040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 245 |
1 |
0 |
|a Epstein-Barr Virus Protocols /
|c edited by Joanna B. Wilson, Gerhard H. W. May.
|
| 250 |
|
|
|a 1st ed. 2001.
|
| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2001.
|
| 300 |
|
|
|a XIV, 438 p. :
|b online resource.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Methods in Molecular Biology,
|v 174
|
| 505 |
0 |
|
|a Genome and Transcript Analyses -- Epstein-Barr Virus -- Analysis of Replication of oriP-Based Plasmids by Quantitative, Competitive PCR -- Genetic Analysis and Gene Expression with Mini-Epstein-Barr Virus Plasmids -- Construction of cDNA Libraries for the Analysis of the Structure of Complementary Strand Transcripts (CSTs) -- Analysis of the Expression and Function of the EBV-Encoded Small RNAs, the EBERs, in Heterologous Cells -- Visualizing EBV Expression Patterns by FISH -- Viral Detection -- In Situ Detection of Epstein-Barr Virus DNA and Viral Gene Products -- Phenotype Determination of Epstein-Barr Virus-Infected Cells in Tissue Sections -- Detection of EBV Infection at the Single-Cell Level -- Detection and Discrimination of Latent and Replicative Herpesvirus Infection at the Single Cell Level In Vivo -- Culture Methods -- Virus Isolation -- Generation of Lymphoblastoid Cell Lines (LCLs) -- Cell Cycle Distribution of B-Lymphocytes and Cell Lines -- of Plasmid Vectors into Cells Via Electroporation -- Malignant Transformation and Immortalization Assays in Animal Cells Transfected with the BARF1 Gene -- Transient Gene Expression and MACS Enrichment -- In Vitro Assays to Study Epithelial Cell Growth -- In Vitro Assays to Study Epithelial Cell Differentiation -- Cell Sensitivity Assays -- Qualitative Detection of Apoptotic Cells Assessed by DNA Fragmentation -- Immune Assays -- Regression Assay -- Generation of Polyclonal EBV-Specific CTL Cultures and Clones -- Determination of Antigen and Fine Peptide Specificity of EBV-Specific CTLs -- Limiting Dilution Assay -- Viral Protein Detection -- Antibodies for Detecting EBV Latent Proteins -- Detection of EBV Latent Proteins by Western Blotting -- Biosynthetic Radiolabeling of Virus Glycoproteins for Immunoprecipitation and Electrophoretic Analysis -- Protein-Protein, Protein-DNA, and Protein-RNA Interactions -- The Yeast Two-Hybrid Assay to Identify Interacting Proteins -- Identification of Transactivation, Repression, and Protein-Protein Interaction Domains Using GAL4-Fusion Proteins -- Magnetic DNA Affinity Purification of a Cellular Transcription Factor -- Screening an Expression Library with a DNA Binding Site -- Analysis of DNA Binding Proteins by Mobility Shift Assay -- Analysis of RNA-Protein Interactions of the EBV-Encoded Small RNAs, the EBERs -- Protein Activity Assays -- Chimeric and Mutated Variants of LMP1 -- Assaying the Activity of Kinases Regulated by LMP1 -- In Vitro Assays for the Detection of Protein Tyrosine Phosphorylation and Protein Tyrosine Kinase Activities -- Tissue and In Vivo Protocols -- Analysis of Apoptosis in Tissue Sections -- Considerations in Generating Transgenic Mice -- In Vivo Assay of Cellular Proliferation -- Topical Chemical Carcinogen Treatment in Mice -- Separation of Epidermal Tissue from Underlying Dermis and Primary Keratinocyte Culture -- Selection and Enrichment of B Cells from Lymphoid Tissues -- Detection of Immunoglobulin Gene Rearrangements.
|
| 650 |
|
0 |
|a Immunology.
|
| 650 |
1 |
4 |
|a Immunology.
|
| 700 |
1 |
|
|a Wilson, Joanna B.
|e editor.
|
| 700 |
1 |
|
|a May, Gerhard H. W.
|e editor.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|