Epstein-Barr Virus Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2001.
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| Edición: | 1st ed. 2001. |
| Colección: | Methods in Molecular Biology,
174 |
| Materias: |
Tabla de Contenidos:
- Genome and Transcript Analyses
- Epstein-Barr Virus
- Analysis of Replication of oriP-Based Plasmids by Quantitative, Competitive PCR
- Genetic Analysis and Gene Expression with Mini-Epstein-Barr Virus Plasmids
- Construction of cDNA Libraries for the Analysis of the Structure of Complementary Strand Transcripts (CSTs)
- Analysis of the Expression and Function of the EBV-Encoded Small RNAs, the EBERs, in Heterologous Cells
- Visualizing EBV Expression Patterns by FISH
- Viral Detection
- In Situ Detection of Epstein-Barr Virus DNA and Viral Gene Products
- Phenotype Determination of Epstein-Barr Virus-Infected Cells in Tissue Sections
- Detection of EBV Infection at the Single-Cell Level
- Detection and Discrimination of Latent and Replicative Herpesvirus Infection at the Single Cell Level In Vivo
- Culture Methods
- Virus Isolation
- Generation of Lymphoblastoid Cell Lines (LCLs)
- Cell Cycle Distribution of B-Lymphocytes and Cell Lines
- of Plasmid Vectors into Cells Via Electroporation
- Malignant Transformation and Immortalization Assays in Animal Cells Transfected with the BARF1 Gene
- Transient Gene Expression and MACS Enrichment
- In Vitro Assays to Study Epithelial Cell Growth
- In Vitro Assays to Study Epithelial Cell Differentiation
- Cell Sensitivity Assays
- Qualitative Detection of Apoptotic Cells Assessed by DNA Fragmentation
- Immune Assays
- Regression Assay
- Generation of Polyclonal EBV-Specific CTL Cultures and Clones
- Determination of Antigen and Fine Peptide Specificity of EBV-Specific CTLs
- Limiting Dilution Assay
- Viral Protein Detection
- Antibodies for Detecting EBV Latent Proteins
- Detection of EBV Latent Proteins by Western Blotting
- Biosynthetic Radiolabeling of Virus Glycoproteins for Immunoprecipitation and Electrophoretic Analysis
- Protein-Protein, Protein-DNA, and Protein-RNA Interactions
- The Yeast Two-Hybrid Assay to Identify Interacting Proteins
- Identification of Transactivation, Repression, and Protein-Protein Interaction Domains Using GAL4-Fusion Proteins
- Magnetic DNA Affinity Purification of a Cellular Transcription Factor
- Screening an Expression Library with a DNA Binding Site
- Analysis of DNA Binding Proteins by Mobility Shift Assay
- Analysis of RNA-Protein Interactions of the EBV-Encoded Small RNAs, the EBERs
- Protein Activity Assays
- Chimeric and Mutated Variants of LMP1
- Assaying the Activity of Kinases Regulated by LMP1
- In Vitro Assays for the Detection of Protein Tyrosine Phosphorylation and Protein Tyrosine Kinase Activities
- Tissue and In Vivo Protocols
- Analysis of Apoptosis in Tissue Sections
- Considerations in Generating Transgenic Mice
- In Vivo Assay of Cellular Proliferation
- Topical Chemical Carcinogen Treatment in Mice
- Separation of Epidermal Tissue from Underlying Dermis and Primary Keratinocyte Culture
- Selection and Enrichment of B Cells from Lymphoid Tissues
- Detection of Immunoglobulin Gene Rearrangements.