|
|
|
|
| LEADER |
02253nam a22002895i 4500 |
| 001 |
000281368 |
| 005 |
20211119151451.0 |
| 007 |
cr nn 008mamaa |
| 008 |
100301s2001 xxu| s |||| 0|eng d |
| 020 |
|
|
|a 9781592592203
|
| 024 |
7 |
|
|a 10.1385/1592592201
|2 doi
|
| 040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
| 245 |
1 |
0 |
|a Gene Knockout Protocols /
|c edited by Martin J. Tymms, Ismail Kola.
|
| 250 |
|
|
|a 1st ed. 2001.
|
| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2001.
|
| 300 |
|
|
|a XI, 431 p. :
|b online resource.
|
| 336 |
|
|
|a text
|b txt
|2 rdacontent
|
| 337 |
|
|
|a computer
|b c
|2 rdamedia
|
| 338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
| 490 |
1 |
|
|a Methods in Molecular Biology,
|v 158
|
| 505 |
0 |
|
|a Overview -- Isolation and Maintenance of Primate Embryonic Stem Cells -- Gene Targeting in ES Cells -- Manipulating Mouse Embryonic Stem Cells -- Gene Targeting in a Centralized Facility -- The LoxP/CRE System and Genome Modification -- Creation and Use of a Cre Recombinase Transgenic Database -- Choice of Mouse Strains for Gene Targeting -- Isolation, Microinjection, and Transfer of Mouse Blastocysts -- Aggregation Chimeras -- How to Study Pathologic Phenotypes of Knockout Mice -- Analysis of Hematopoietic Phenotypes in Knockout Mouse Models -- Isolation of Embryonic Fibroblasts and Their Use in the In Vitro Characterization of Gene Function -- Influence of Genetic Background on Knockout Mouse Phenotypes -- Lineage Allocation During Early Embryogenesis -- Generation of Double-Knockout Embryonic Stem Cells -- In Vitro Differentiation of Embryonic Stem Cells and Analysis of Cellular Phenotypes -- Embryonic Stem Cells in the Study of Hematopoiesis -- Interferon-Inducible ES Cell Expression Systems -- Transgenic Studies in the Mouse -- In Vivo Libraries of Large Insert Transgenic Mice for Genetic Mapping -- Epigenetic Effects on Transgene Expression -- Positional-Candidate Cloning of Genes from Mouse Mutants -- Genetically Engineered Mice -- Embryo Cryopreservation for Transgenic Mouse Lines.
|
| 650 |
|
0 |
|a Human genetics.
|
| 650 |
1 |
4 |
|a Human Genetics.
|
| 700 |
1 |
|
|a Tymms, Martin J.
|e editor.
|
| 700 |
1 |
|
|a Kola, Ismail.
|e editor.
|
| 710 |
2 |
|
|a SpringerLink (Online service)
|
| 773 |
0 |
|
|t Springer eBooks
|