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02252nam a22002895i 4500 |
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100301s2001 xxu| s |||| 0|eng d |
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|a 9781592592203
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024 |
7 |
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|a 10.1385/1592592201
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
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|a Gene Knockout Protocols /
|c edited by Martin J. Tymms, Ismail Kola.
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250 |
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|a 1st ed. 2001.
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260 |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2001.
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|a XI, 431 p. :
|b online resource.
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 158
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|a Overview -- Isolation and Maintenance of Primate Embryonic Stem Cells -- Gene Targeting in ES Cells -- Manipulating Mouse Embryonic Stem Cells -- Gene Targeting in a Centralized Facility -- The LoxP/CRE System and Genome Modification -- Creation and Use of a Cre Recombinase Transgenic Database -- Choice of Mouse Strains for Gene Targeting -- Isolation, Microinjection, and Transfer of Mouse Blastocysts -- Aggregation Chimeras -- How to Study Pathologic Phenotypes of Knockout Mice -- Analysis of Hematopoietic Phenotypes in Knockout Mouse Models -- Isolation of Embryonic Fibroblasts and Their Use in the In Vitro Characterization of Gene Function -- Influence of Genetic Background on Knockout Mouse Phenotypes -- Lineage Allocation During Early Embryogenesis -- Generation of Double-Knockout Embryonic Stem Cells -- In Vitro Differentiation of Embryonic Stem Cells and Analysis of Cellular Phenotypes -- Embryonic Stem Cells in the Study of Hematopoiesis -- Interferon-Inducible ES Cell Expression Systems -- Transgenic Studies in the Mouse -- In Vivo Libraries of Large Insert Transgenic Mice for Genetic Mapping -- Epigenetic Effects on Transgene Expression -- Positional-Candidate Cloning of Genes from Mouse Mutants -- Genetically Engineered Mice -- Embryo Cryopreservation for Transgenic Mouse Lines.
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|a Human genetics.
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650 |
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|a Human Genetics.
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|a Tymms, Martin J.
|e editor.
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|a Kola, Ismail.
|e editor.
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710 |
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|a SpringerLink (Online service)
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773 |
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|t Springer eBooks
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