Gene Knockout Protocols /
| Autor Corporativo: | |
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| Otros Autores: | , |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2001.
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| Edición: | 1st ed. 2001. |
| Colección: | Methods in Molecular Biology,
158 |
| Materias: |
Tabla de Contenidos:
- Overview
- Isolation and Maintenance of Primate Embryonic Stem Cells
- Gene Targeting in ES Cells
- Manipulating Mouse Embryonic Stem Cells
- Gene Targeting in a Centralized Facility
- The LoxP/CRE System and Genome Modification
- Creation and Use of a Cre Recombinase Transgenic Database
- Choice of Mouse Strains for Gene Targeting
- Isolation, Microinjection, and Transfer of Mouse Blastocysts
- Aggregation Chimeras
- How to Study Pathologic Phenotypes of Knockout Mice
- Analysis of Hematopoietic Phenotypes in Knockout Mouse Models
- Isolation of Embryonic Fibroblasts and Their Use in the In Vitro Characterization of Gene Function
- Influence of Genetic Background on Knockout Mouse Phenotypes
- Lineage Allocation During Early Embryogenesis
- Generation of Double-Knockout Embryonic Stem Cells
- In Vitro Differentiation of Embryonic Stem Cells and Analysis of Cellular Phenotypes
- Embryonic Stem Cells in the Study of Hematopoiesis
- Interferon-Inducible ES Cell Expression Systems
- Transgenic Studies in the Mouse
- In Vivo Libraries of Large Insert Transgenic Mice for Genetic Mapping
- Epigenetic Effects on Transgene Expression
- Positional-Candidate Cloning of Genes from Mouse Mutants
- Genetically Engineered Mice
- Embryo Cryopreservation for Transgenic Mouse Lines.