Food Allergens : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Lin, Jing. (Editor ), Alcocer, Marcos. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1592
Materias:
Tabla de Contenidos:
  • Overview of the Commonly used Methods for Food Allergens
  • Allergen Extraction and Purification from Natural Products: Main Chromatographic Techniques
  • Recombinant Allergen Production in E. Coli
  • Recombinant Allergens Production in Yeast
  • 2D-Electrophoresis and Immunoblotting in Food Allergy
  • Two Dimensional Electrophoresis and Identification by Mass Spectrometry (MS)
  • Enzyme-Linked Immunosorbent Assay (ELISA)
  • Detection of Food Allergens by Taqman Real-Time PCR Methodology
  • Detection of Food Allergens by Phage Displayed Produced Antibodies
  • Protein Microarray-Based IgE-Immunoassay for Allergy Diagnosis
  • Basophil Degranulation Assay
  • Use of Humanized RS-ATL8 Reporter System for Detection of Allergen-Specific IgE Sensitization in Human Food Allergy
  • Assessment of IgE Reactivity of β-Casein by Western Blotting after Digestion with Simulated Gastric Fluid
  • IgE Epitope Mapping using Peptide Microarray Immunoassay
  • T Cell Proliferation Assay: Determination of Immunodominant T Cell Epitopes of Food Allergens
  • Tetramer Guided Epitope Mapping: A Rapid Approach to Identify HLA-Restricted T-Cell Epitopes from Composite Allergens
  • T-Cell Epitope Prediction
  • An Overview of Bioinformatics Tools and Resources in Allergy
  • The Use of a Semi-Automated System to Measure Mouse Natural Killer T (NKT) Cell Activation by Lipid Loaded Dendritic Cells
  • Recent Advances in the Detection of Allergens in Foods.