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170823s2017 xxu| s |||| 0|eng d |
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|a 9781493971138
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024 |
7 |
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|a 10.1007/978-1-4939-7113-8
|2 doi
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040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
1 |
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|a Fibrosis :
|b Methods and Protocols /
|c edited by Laure Rittié.
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250 |
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|a 1st ed. 2017.
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260 |
# |
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|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2017.
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300 |
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|a XV, 530 p. 170 illus., 139 illus. in color. :
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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337 |
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|a computer
|b c
|2 rdamedia
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338 |
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|a online resource
|b cr
|2 rdacarrier
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490 |
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|a Methods in Molecular Biology,
|v 1627
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|a Human Fibrotic Diseases: Current Challenges in Fibrosis Research -- The Bleomycin Model of Pulmonary Fibrosis -- Intra-Dermal Injections of Bleomycin to Model Skin Fibrosis -- Assessing the Effects of Fibrosis on Lung Function by Light Microscopy-Coupled Stereology -- Transplanting Human Skin Grafts onto Nude Mice to Model Skin Scars -- Hypertrophic Scarring in the Rabbit Ear: A Practical Model for Studying Dermal Fibrosis -- Mouse and Rat Models of Induction of Hepatic Fibrosis and Assessment of Portal Hypertension -- Mouse Models of Corneal Scarring -- Modeling Cardiac Fibrosis in Mice: (Myo)Fibroblast Phenotype after Ischemia -- Characterization of Mesenchymal-Fibroblast Cells using the Col1a2 Promoter/Enhancer -- Isolation and Culture of Primary Murine Hepatic Stellate Cells -- Isolation and Culture of Adipose-Derived Stromal Cells from Sub-Cutaneous Fat -- Isolation of Live Fibroblasts by Fluorescence-Activated Cell Sorting -- Detection of Infiltrating Mast Cells using a Modified Toluidine Blue Staining -- Cell-Populated Collagen Lattice Models -- Traction Force Measurement using Deformable Microposts -- Mechanical Deformation of Cultured Cells with Hydrogels -- Preparation of Decellularized Lung Matrices for Cell Culture and Protein Analysis -- Type I Collagen Purification from Rat Tail Tendons -- Purification of Human Plasma/Cellular Fibronectin and Fibronectin Fragments -- Laser Capture Microdissection of Tissue Sections for High-Throughput RNA Analysis -- Collagen Quantification in Tissue Specimens -- Methods for the Assessment of Active Transforming Growth Factor-β in Cells and Tissues -- Visualizing in Vitro Type I Collagen Fibrillogenesis by Transmission Electron Microscopy -- Histological and Electron Microscope Staining for the Identification of Elastic Fiber Networks -- Method for Picrosirius Red-Polarization Detection of Collagen Fibers in Tissue Sections -- Probing Collagen Organization: Practical Guide for Second Harmonic Generation (SHG) Imaging -- Methods for Quantifying Fibrillar Collagen Alignment -- Exploring the Nano-Surface of Collagenous and Other Fibrotic Tissues with AFM -- Spectral Unmixing Methods and Tools for the Detection and Quantitation of Collagen and Other Macromolecules in Tissue Specimens -- Simple Analysis of Deposited Gene Expression Datasets for the Non-Bioinformatician: How to Use GEO for Fibrosis Research.
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650 |
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|a Molecular biology.
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650 |
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4 |
|a Molecular Medicine.
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700 |
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|a Rittié, Laure.
|e editor.
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710 |
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|a SpringerLink (Online service)
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773 |
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|t Springer eBooks
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900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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