Fibrosis : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Rittié, Laure. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1627
Materias:
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245 1 0 |a Fibrosis :  |b Methods and Protocols /  |c edited by Laure Rittié. 
250 |a 1st ed. 2017. 
260 # # |a New York, NY :  |b Springer New York :  |b Imprint: Humana,  |c 2017. 
300 |a XV, 530 p. 170 illus., 139 illus. in color. :  |b online resource. 
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490 1 |a Methods in Molecular Biology,  |v 1627 
505 0 |a Human Fibrotic Diseases: Current Challenges in Fibrosis Research -- The Bleomycin Model of Pulmonary Fibrosis -- Intra-Dermal Injections of Bleomycin to Model Skin Fibrosis -- Assessing the Effects of Fibrosis on Lung Function by Light Microscopy-Coupled Stereology -- Transplanting Human Skin Grafts onto Nude Mice to Model Skin Scars -- Hypertrophic Scarring in the Rabbit Ear: A Practical Model for Studying Dermal Fibrosis -- Mouse and Rat Models of Induction of Hepatic Fibrosis and Assessment of Portal Hypertension -- Mouse Models of Corneal Scarring -- Modeling Cardiac Fibrosis in Mice: (Myo)Fibroblast Phenotype after Ischemia -- Characterization of Mesenchymal-Fibroblast Cells using the Col1a2 Promoter/Enhancer -- Isolation and Culture of Primary Murine Hepatic Stellate Cells -- Isolation and Culture of Adipose-Derived Stromal Cells from Sub-Cutaneous Fat -- Isolation of Live Fibroblasts by Fluorescence-Activated Cell Sorting -- Detection of Infiltrating Mast Cells using a Modified Toluidine Blue Staining -- Cell-Populated Collagen Lattice Models -- Traction Force Measurement using Deformable Microposts -- Mechanical Deformation of Cultured Cells with Hydrogels -- Preparation of Decellularized Lung Matrices for Cell Culture and Protein Analysis -- Type I Collagen Purification from Rat Tail Tendons -- Purification of Human Plasma/Cellular Fibronectin and Fibronectin Fragments -- Laser Capture Microdissection of Tissue Sections for High-Throughput RNA Analysis -- Collagen Quantification in Tissue Specimens -- Methods for the Assessment of Active Transforming Growth Factor-β in Cells and Tissues -- Visualizing in Vitro Type I Collagen Fibrillogenesis by Transmission Electron Microscopy -- Histological and Electron Microscope Staining for the Identification of Elastic Fiber Networks -- Method for Picrosirius Red-Polarization Detection of Collagen Fibers in Tissue Sections -- Probing Collagen Organization: Practical Guide for Second Harmonic Generation (SHG) Imaging -- Methods for Quantifying Fibrillar Collagen Alignment -- Exploring the Nano-Surface of Collagenous and Other Fibrotic Tissues with AFM -- Spectral Unmixing Methods and Tools for the Detection and Quantitation of Collagen and Other Macromolecules in Tissue Specimens -- Simple Analysis of Deposited Gene Expression Datasets for the Non-Bioinformatician: How to Use GEO for Fibrosis Research. 
650 0 |a Molecular biology. 
650 1 4 |a Molecular Medicine. 
700 1 |a Rittié, Laure.  |e editor. 
710 2 |a SpringerLink (Online service) 
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