Fibrosis : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Rittié, Laure. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2017.
Edición:1st ed. 2017.
Colección:Methods in Molecular Biology, 1627
Materias:
Tabla de Contenidos:
  • Human Fibrotic Diseases: Current Challenges in Fibrosis Research
  • The Bleomycin Model of Pulmonary Fibrosis
  • Intra-Dermal Injections of Bleomycin to Model Skin Fibrosis
  • Assessing the Effects of Fibrosis on Lung Function by Light Microscopy-Coupled Stereology
  • Transplanting Human Skin Grafts onto Nude Mice to Model Skin Scars
  • Hypertrophic Scarring in the Rabbit Ear: A Practical Model for Studying Dermal Fibrosis
  • Mouse and Rat Models of Induction of Hepatic Fibrosis and Assessment of Portal Hypertension
  • Mouse Models of Corneal Scarring
  • Modeling Cardiac Fibrosis in Mice: (Myo)Fibroblast Phenotype after Ischemia
  • Characterization of Mesenchymal-Fibroblast Cells using the Col1a2 Promoter/Enhancer
  • Isolation and Culture of Primary Murine Hepatic Stellate Cells
  • Isolation and Culture of Adipose-Derived Stromal Cells from Sub-Cutaneous Fat
  • Isolation of Live Fibroblasts by Fluorescence-Activated Cell Sorting
  • Detection of Infiltrating Mast Cells using a Modified Toluidine Blue Staining
  • Cell-Populated Collagen Lattice Models
  • Traction Force Measurement using Deformable Microposts
  • Mechanical Deformation of Cultured Cells with Hydrogels
  • Preparation of Decellularized Lung Matrices for Cell Culture and Protein Analysis
  • Type I Collagen Purification from Rat Tail Tendons
  • Purification of Human Plasma/Cellular Fibronectin and Fibronectin Fragments
  • Laser Capture Microdissection of Tissue Sections for High-Throughput RNA Analysis
  • Collagen Quantification in Tissue Specimens
  • Methods for the Assessment of Active Transforming Growth Factor-β in Cells and Tissues
  • Visualizing in Vitro Type I Collagen Fibrillogenesis by Transmission Electron Microscopy
  • Histological and Electron Microscope Staining for the Identification of Elastic Fiber Networks
  • Method for Picrosirius Red-Polarization Detection of Collagen Fibers in Tissue Sections
  • Probing Collagen Organization: Practical Guide for Second Harmonic Generation (SHG) Imaging
  • Methods for Quantifying Fibrillar Collagen Alignment
  • Exploring the Nano-Surface of Collagenous and Other Fibrotic Tissues with AFM
  • Spectral Unmixing Methods and Tools for the Detection and Quantitation of Collagen and Other Macromolecules in Tissue Specimens
  • Simple Analysis of Deposited Gene Expression Datasets for the Non-Bioinformatician: How to Use GEO for Fibrosis Research.