|
|
|
|
LEADER |
04368nam a22003135i 4500 |
001 |
000281615 |
005 |
20210506140053.0 |
007 |
cr nn 008mamaa |
008 |
130228s2013 xxu| s |||| 0|eng d |
020 |
|
|
|a 9781627032841
|
024 |
7 |
|
|a 10.1007/978-1-62703-284-1
|2 doi
|
040 |
|
|
|a Sistema de Bibliotecas del Tecnológico de Costa Rica
|
245 |
1 |
0 |
|a Gene Regulation :
|b Methods and Protocols /
|c edited by Minou Bina.
|
250 |
|
|
|a 1st ed. 2013.
|
260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2013.
|
300 |
|
|
|a XV, 401 p. 80 illus., 19 illus. in color. :
|b online resource.
|
336 |
|
|
|a text
|b txt
|2 rdacontent
|
337 |
|
|
|a computer
|b c
|2 rdamedia
|
338 |
|
|
|a online resource
|b cr
|2 rdacarrier
|
490 |
1 |
|
|a Methods in Molecular Biology,
|v 977
|
505 |
0 |
|
|a Gene Regulation -- Isolation of Nuclei for use in Genome-wide DNase Hypersensitivity Assays to Probe Chromatin Structure -- DNase I Digestion of Isolated Nulcei for Genome-Wide Mapping of DNase Hypersensitivity Sites in Chromatin -- Isolation and Analysis of DNA Derived from Nucleosome-Free Regions -- Acquisition of High Quality DNA for Massive Parallel Sequencing by in vivo Chromatin Immunoprecipitation -- Luciferase Assay to Study the Activity of a Cloned Promoter DNA Fragment -- Promoter Deletion Analysis using a Dual-luciferase Reporter System -- Application of mRNA Display for in vitro Selection of DNA-binding Transcription Factor Complexes -- Isolation of Intracellular Protein:DNA Complexes using HaloCHIP; An Antibody Free Alternative to Chromatin Immunoprecipitation -- A Modified Yeast One-Hybrid System for Genome Wide Identification of Transcription Factor Binding Sites -- Identifying Specific Protein--DNA Interactions using SILAC--based Quantitative Proteomics -- Electrophoretic Mobility-Shift and Super-Shift Assays for Studies and Characterization of Protein-DNA Complexes -- Combination of Native and Denaturing PAGE for the Detection of Protein Binding Regions in Long Fragments of Genomic DNA -- Quantitative NanoProteomics Approach for Protein Complex (QNanoPX) using Gold Nanoparticle-based DNA Probe -- Chromatin Assembly and In Vitro Transcription Analyses for Evaluation of Individual Protein Activities in Multicomponent Transcriptional Complexes -- Using FRET to Monitor Protein-induced DNA Bending: The TBP-TATA Complex as a Model System -- Promoter Independent Abortive Transcription Assays Unravel Functional Interactions Between TFIIB and RNA Polymerase -- Fluorescence Cross-correlation Spectroscopy (FCCS) to Observe Dimerization of Transcription Factors in Living Cells -- Nuclear Recruitment Assay as a Tool to validate Transcription Factor Interactions in Mammalian Cells -- Preparation of Cell Lines for Single-Cell Analysis of Transcriptional Activation Dynamics -- Peptide Microarrays for Profiling of Serine/threonine Kinase Activity of Recombinant Kinases and Lysates of Cells and Tissue Samples -- Immunoaffinity Purification of Protein Complexes from Mammalian Cells -- Simple and Efficient Identification of Chromatin Modifying Complexes and Characterization of Complex Composition -- Heavy Methyl-SILAC Labeling Coupled with Liquid Chromatography and High-resolution Mass Spectrometry to Study the Dynamics of Site-specific Histone Methylation -- Analysis of p300 Occupancy at the Early Stage of Stem Cell Differentiation by Chromatin Immunoprecipitation -- Mammalian Two-Hybrid Assays for Studies of Interaction of p300 with Transcription Factors -- Fluorescence Anisotropy Microplate Assay to Investigate the Interaction of Full-length Steroid Receptor Coactivator-1a with Steroid Receptors -- Use of Histone Deacetylase Inhibitors to Examine the Roles of Bromodomain and Histone Acetylation in p300-dependent Gene Expression -- Histone Deacetylases Valproic Acid as a Small Molecule Inducer to Direct the Differentiation of Pluripotent Stem Cells -- Sedimentation and Immunoprecipitation Assays for Analyzing Complexes that Repress Transcription -- Methods for Studies of Protein Interactions with Different DNA Methyltransferases.
|
650 |
|
0 |
|a Human genetics.
|
650 |
|
0 |
|a Gene expression.
|
650 |
1 |
4 |
|a Human Genetics.
|
650 |
2 |
4 |
|a Gene Expression.
|
700 |
1 |
|
|a Bina, Minou.
|e editor.
|
710 |
2 |
|
|a SpringerLink (Online service)
|
773 |
0 |
|
|t Springer eBooks
|
900 |
|
|
|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
|