Gene Regulation : Methods and Protocols /
| Autor Corporativo: | |
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| Otros Autores: | |
| Formato: | eBook |
| Lenguaje: | English |
| Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2013.
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| Edición: | 1st ed. 2013. |
| Colección: | Methods in Molecular Biology,
977 |
| Materias: |
Tabla de Contenidos:
- Gene Regulation
- Isolation of Nuclei for use in Genome-wide DNase Hypersensitivity Assays to Probe Chromatin Structure
- DNase I Digestion of Isolated Nulcei for Genome-Wide Mapping of DNase Hypersensitivity Sites in Chromatin
- Isolation and Analysis of DNA Derived from Nucleosome-Free Regions
- Acquisition of High Quality DNA for Massive Parallel Sequencing by in vivo Chromatin Immunoprecipitation
- Luciferase Assay to Study the Activity of a Cloned Promoter DNA Fragment
- Promoter Deletion Analysis using a Dual-luciferase Reporter System
- Application of mRNA Display for in vitro Selection of DNA-binding Transcription Factor Complexes
- Isolation of Intracellular Protein:DNA Complexes using HaloCHIP; An Antibody Free Alternative to Chromatin Immunoprecipitation
- A Modified Yeast One-Hybrid System for Genome Wide Identification of Transcription Factor Binding Sites
- Identifying Specific Protein
- DNA Interactions using SILAC
- based Quantitative Proteomics
- Electrophoretic Mobility-Shift and Super-Shift Assays for Studies and Characterization of Protein-DNA Complexes
- Combination of Native and Denaturing PAGE for the Detection of Protein Binding Regions in Long Fragments of Genomic DNA
- Quantitative NanoProteomics Approach for Protein Complex (QNanoPX) using Gold Nanoparticle-based DNA Probe
- Chromatin Assembly and In Vitro Transcription Analyses for Evaluation of Individual Protein Activities in Multicomponent Transcriptional Complexes
- Using FRET to Monitor Protein-induced DNA Bending: The TBP-TATA Complex as a Model System
- Promoter Independent Abortive Transcription Assays Unravel Functional Interactions Between TFIIB and RNA Polymerase
- Fluorescence Cross-correlation Spectroscopy (FCCS) to Observe Dimerization of Transcription Factors in Living Cells
- Nuclear Recruitment Assay as a Tool to validate Transcription Factor Interactions in Mammalian Cells
- Preparation of Cell Lines for Single-Cell Analysis of Transcriptional Activation Dynamics
- Peptide Microarrays for Profiling of Serine/threonine Kinase Activity of Recombinant Kinases and Lysates of Cells and Tissue Samples
- Immunoaffinity Purification of Protein Complexes from Mammalian Cells
- Simple and Efficient Identification of Chromatin Modifying Complexes and Characterization of Complex Composition
- Heavy Methyl-SILAC Labeling Coupled with Liquid Chromatography and High-resolution Mass Spectrometry to Study the Dynamics of Site-specific Histone Methylation
- Analysis of p300 Occupancy at the Early Stage of Stem Cell Differentiation by Chromatin Immunoprecipitation
- Mammalian Two-Hybrid Assays for Studies of Interaction of p300 with Transcription Factors
- Fluorescence Anisotropy Microplate Assay to Investigate the Interaction of Full-length Steroid Receptor Coactivator-1a with Steroid Receptors
- Use of Histone Deacetylase Inhibitors to Examine the Roles of Bromodomain and Histone Acetylation in p300-dependent Gene Expression
- Histone Deacetylases Valproic Acid as a Small Molecule Inducer to Direct the Differentiation of Pluripotent Stem Cells
- Sedimentation and Immunoprecipitation Assays for Analyzing Complexes that Repress Transcription
- Methods for Studies of Protein Interactions with Different DNA Methyltransferases.