Functional Analysis of DNA and Chromatin /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Stockert, Juan C. (Editor ), Espada, Jesús. (Editor ), Blázquez-Castro, Alfonso. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1094
Materias:
Tabla de Contenidos:
  • Predictive Binding Geometry of Ligands to DNA Minor Groove: Isohelicity and Hydrogen-Bonding Pattern
  • Using Microchip Gel Electrophoresis to Probe DNA-Drug Binding Interactions
  • Identifying Different Types of Chromatin using Giemsa Staining
  • Analysis of DNA Damage and Repair by Comet Fluorescence in situ Hybridization (Comet-FISH)
  • Alkaline Nuclear Dispersion Assays for the Determination of DNA Damage at the Single Cell Level
  • Polarization Microscopy of Extended Chromatin Fibres
  • DNA Labeling in Vivo: Quantification of Epidermal Stem Cell Chromatin Content in Whole Mouse Hair Follicles Using Fiji Image Processing Software
  • A Historical Overview of Bromo-Substituted DNA and Sister Chromatid Differentiation
  • Image Analysis of Chromatin Remodelling
  • FISH Methods in Cytogenetic Studies
  • Ultrastructural and Immunofluorescent Methods for the Study of the XY Body as a Biomarker
  • Atomic Force Microscopy for Analyzing Metaphase Chromosomes. Comparison of AFM Images with Fluorescence Labeling Images of Banding Patterns
  • Selective Detection of Phagocytic Phase of Apoptosis in Fixed Tissue Sections
  • Transport of Cationized Fluorescent Topoisomerase into Nuclei of Live Cells for DNA Damage Studies
  • Visualization and Interpretation of Eukaryotic DNA Replication Intermediates in vivo by Electron Microscopy
  • Combined Bi-dimensional Electrophoresis and Electron Microscopy to Study Specific Plasmid DNA Replication Intermediates in Human Cells
  • Standard DNA Methylation Analysis in Mouse Epidermis: Bisulfite Sequencing, Methylation-Specific PCR, and 5-Methyl-Cytosine (5mC) Immunological Detection
  • Methyl-combing—Single Molecule Analysis of DNA Methylation on Stretched DNA Fibers
  • Investigating 5-Hydroxymethylcytosine (5hmC): The State of the Art
  • Hydroxymethylated DNA Immunoprecipitation (hmeDIP)
  • Microscale Thermophoresis for the Assessment of Nuclear Protein Binding Affinities
  • Analysis of Histone Post-translational Modifications from Nucleolus-associated Chromatin by Mass Spectrometry
  • Salt-Urea, Sulfopropyl-Sepharose, and Covalent Chromatography Methods for Histone Isolation and Fractionation
  • Chromatin Immunoprecipitation
  • Analysis of Chromatin Composition of Repetitive Sequences: the ChIP-chop Assay
  • Purification of Specific Chromatin Domains from Single-Copy Gene Loci in Saccharomyces cerevisiae
  • Deep Sequencing of Small Chromatin-Associated RNA – Isolation and Library Preparation
  • Deep Sequencing of Small Chromatin-Associated RNA – Bioinformatic Analysis.