Connexin Methods and Protocols /
| Corporate Author: | |
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| Other Authors: | , |
| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2001.
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| Edition: | 1st ed. 2001. |
| Series: | Methods in Molecular Biology,
154 |
| Subjects: |
Table of Contents:
- Tools to Study Connexins
- Investigation of Connexin Gene Expression Patterns by In Situ Hybridization Techniques
- Sodium Dodecyl Sulfate-Freeze-Fracture Immunolabeling of Gap Junctions
- Purification of Gap Junctions
- Culturing of Mammalian Cells Expressing Recombinant Connexins and Two-Dimensional Crystallization of the Isolated Gap Junctions
- Connexins/Connexons
- Biochemical Analysis of Connexon Assembly
- Expression and Imaging of Connexin-GFP Chimeras in Live Mammalian Cells
- Analysis of Connexin Expression in Brain Slices by Single-Cell Reverse Transcriptase Polymerase Chain Reaction
- Use of Retroviruses to Express Connexins
- Spatiotemporal Depletion of Connexins Using Antisense Oligonucleotides
- Transfection and Expression of Exogenous Connexins in Mammalian Cells
- Assays For Function
- Assaying the Molecular Permeability of Connexin Channels
- Applying the Xenopus Oocyte Expression System to the Analysis of Gap Junction Proteins
- Mutagenesis to Study Channel Structure
- Dual Patch Clamp
- Determining Ionic Permeabilities of Gap Junction Channels
- Fluorescence Recovery After Photobleaching
- Capture of Transjunctional Metabolites
- Physiology and Biology of Connexins
- The Study of Connexin Hemichannels (Connexons) in Xenopus Oocytes
- Exploring Hemichannel Permeability In Vitro
- Inducing De Novo Formation of Gap Juntion Channels
- Recording and Analysis of Putative Direct Electrical Interactions in the Mammalian Brain
- Intercellular Calcium Signaling and Flash Photolysis of Caged Compounds
- Biochemical Analysis of Connexin Phosphorylation
- How to Close a Gap Junction Channel.