Cytochrome P450 Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Phillips, Ian R. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2006.
Edición:2nd ed. 2006.
Colección:Methods in Molecular Biology, 320
Materias:
Tabla de Contenidos:
  • Cytochrome P450 Nomenclature, 2004
  • Spectral Analyses of Cytochromes P450
  • Establishment of Functional Human Cytochrome P450 Monooxygenase Systems in Escherichia coli
  • Purification of Cytochromes P450
  • Expression of Recombinant Flavin-Containing Monooxygenases in a Baculovirus/Insect Cell System
  • Cytochrome P450 Reconstitution Systems
  • Catalytic Assays for Human Cytochrome P450
  • Enzymatic Analysis of cDNA-Expressed Human CYP1A1, CYP1A2, and CYP1B1 With 7-Ethoxyresorufin as Substrate
  • Spectrofluorometric Analysis of CYP2A6-Catalyzed Coumarin 7-Hydroxylation
  • Determination of CYP2B6 Component of 7-Ethoxy-4-Trifluoromethylcoumarin O-Deethylation Activity in Human Liver Microsomes
  • High-Performance Liquid Chromatography Analysis of CYP2C8-Catalyzed Paclitaxel 6?-Hydroxylation
  • Determination of CYP2C9-Catalyzed Diclofenac 4?-Hydroxylation by High-Performance Liquid Chromatography
  • CYP2C19-Mediated (S)-Mephenytoin 4?-Hydroxylation Assayed by High-Performance Liquid Chromatography With Radiometric Detection
  • CYP2D6-Dependent Bufuralol 1?-Hydroxylation Assayed by Reverse-Phase Ion-Pair High-Performance Liquid Chromatography With Fluorescence Detection
  • Spectrophotometric Analysis of Human CYP2E1-Catalyzed p-Nitrophenol Hydroxylation
  • Thin-Layer Chromatography Analysis of Human CYP3A-Catalyzed Testosterone 6?-Hydroxylation
  • Determination of CYP4A11-Catalyzed Lauric Acid 12-Hydroxylation by High-Performance Liquid Chromatography With Radiometric Detection
  • An Isocratic High-Performance Liquid Chromatography Assay for CYP7A1-Catalyzed Cholesterol 7?-Hydroxylation
  • Use of 7-Ethoxycoumarin to Monitor Multiple Enzymes in the Human CYP1, CYP2, and CYP3 Families
  • Benzydamine N-Oxygenation as a Measure of Flavin-Containing Monooxygenase Activity
  • Prochiral Sulfoxidation as a Probe for Flavin-Containing Monooxygenases
  • Targeting Antipeptide Antibodies Toward Cytochrome P450 Enzymes
  • The Human Cytochrome P450 Allele Nomenclature Committee Web Site
  • Genotyping for Cytochrome P450 Polymorphisms
  • Hepatocyte Cultures in Drug Metabolism and Toxicological Research and Testing
  • Isolation of Rat Hepatocytes
  • Rat Hepatocyte Cultures
  • Rat Hepatocyte Cultures
  • Hepatocytes in Suspension
  • Isolation of Rat Bone Marrow Stem Cells
  • Transfection of Primary Cultures of Rat Hepatocytes
  • Human Hepatocyte Culture
  • Determination of Cellular Localization of Expression of Flavin-Containing Monooxygenase Genes in Mouse Tissues by In Situ Hybridization
  • Deletion of Genes From the Mouse Genome Using Cre/loxP Technology
  • Characterization of Targeted Mouse Embryonic Stem Cell Chromosomes
  • Microinjection of Targeted Embryonic Stem Cells and Establishment of Knockout Mouse Lines for Fmo Genes
  • Use of Reporter Genes to Measure Xenobiotic-Mediated Activation of CYP Gene Transcription.