DNA Replication : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Vengrova, Sonya. (Editor ), Dalgaard, Jacob Z. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 521
Materias:
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020 |a 9781603278157 
024 7 |a 10.1007/978-1-60327-815-7  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a DNA Replication :  |b Methods and Protocols /  |c edited by Sonya Vengrova, Jacob Z. Dalgaard. 
250 |a 1st ed. 2009. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2009. 
300 |a XIV, 692 p. 90 illus., 8 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 521 
505 0 |a Reviews -- DNA Replication Initiation -- DNA Replication Fork Proteins -- Random and Site-Specific Replication Termination -- Checkpoint Regulation of DNA Replication -- to Molecular Combing: Genomics, DNA Replication, and Cancer -- Methods for Analysis of Replication of a Single Locus -- Replication Initiation Point Mapping: Approach and Implications -- Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis -- Topological Analysis of Plasmid DNA Replication Intermediates Using Two-Dimensional Agarose Gels -- Analysis of Telomeric DNA Replication Using Neutral–Alkaline Two-Dimensional Gel Electrophoresis -- Chromatin Immunoprecipitation of Replication Factors Moving with the Replication Fork -- Density Transfer as a Method to Analyze the Progression of DNA Replication Forks -- High-Resolution Mapping of Points of Site-Specific Replication Stalling -- DNA Replication in Nucleus-Free Xenopus Egg Extracts -- Genome-Wide Analysis Methods -- ChIP-Chip to Analyze the Binding of Replication Proteins to Chromatin Using Oligonucleotide DNA Microarrays -- Analyzing Origin Activation Patterns by Copy Number Change Experiments -- Detection of Replication Origins Using Comparative Genomics and Recombinational ARS Assay -- Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes -- Application of Alkaline Sucrose Gradient Centrifugation in the Analysis of DNA Replication After DNA Damage -- Biochemistry and Biophysics Methods -- Isolation of Recombinant DNA Elongation Proteins -- In Vitro Assays for Studying Helicase Activities -- The Use of 2-Aminopurine Fluorescence to Study DNA Polymerase Function -- Single-Molecule Observation of Prokaryotic DNA Replication -- Cell Biology and Genetic Methods -- Visualization of DNA Replication Sites in Mammalian Nuclei -- Cell-Cycle Synchrony for Analysis of S. pombe DNA Replication -- Measuring DNA Content by Flow Cytometry in Fission Yeast -- Microscopy Techniques to Examine DNA Replication in Fission Yeast -- Using the DHFR Heat-Inducible Degron for Protein Inactivation in Schizosaccharomyces pombe -- Assays Used to Study the DNA Replication Checkpoint in Fission Yeast -- Incorporation of Thymidine Analogs for Studying Replication Kinetics in Fission Yeast -- The Fast-Halo Assay for the Assessment of DNA Damage at the Single-Cell Level -- Monitoring Homologous Recombination Following Replication Fork Perturbation in the Fission Yeast Schizosaccharomyces pombe -- DNA Fiber Analysis Methods -- Computational Methods to Study Kinetics of DNA Replication -- Use of DNA Combing to Study DNA Replicationin Xenopus and Human Cell-Free Systems -- Electron Microscopy Methods for Studying In Vivo DNA Replication Intermediates -- Determining the Replication Dynamics of Specific Gene Loci by Single-Molecule Analysis of Replicated DNA -- Use of DNA Combing for Studying DNA Replication In Vivo in Yeast and Mammalian Cells. 
650 0 |a Biochemistry. 
650 0 |a Genetic engineering. 
650 0 |a Animal genetics. 
650 0 |a Biochemical engineering. 
650 1 4 |a Biochemistry, general. 
650 2 4 |a Genetic Engineering. 
650 2 4 |a Animal Genetics and Genomics. 
650 2 4 |a Biochemical Engineering. 
700 1 |a Vengrova, Sonya.  |e editor. 
700 1 |a Dalgaard, Jacob Z.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks