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04229nam a22003615i 4500 |
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100715s2009 xxu| s |||| 0|eng d |
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|a 9781603278157
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024 |
7 |
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|a 10.1007/978-1-60327-815-7
|2 doi
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040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
1 |
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|a DNA Replication :
|b Methods and Protocols /
|c edited by Sonya Vengrova, Jacob Z. Dalgaard.
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250 |
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|a 1st ed. 2009.
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260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
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300 |
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|a XIV, 692 p. 90 illus., 8 illus. in color. :
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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338 |
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|a online resource
|b cr
|2 rdacarrier
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490 |
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|a Methods in Molecular Biology,
|v 521
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505 |
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|a Reviews -- DNA Replication Initiation -- DNA Replication Fork Proteins -- Random and Site-Specific Replication Termination -- Checkpoint Regulation of DNA Replication -- to Molecular Combing: Genomics, DNA Replication, and Cancer -- Methods for Analysis of Replication of a Single Locus -- Replication Initiation Point Mapping: Approach and Implications -- Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis -- Topological Analysis of Plasmid DNA Replication Intermediates Using Two-Dimensional Agarose Gels -- Analysis of Telomeric DNA Replication Using Neutral–Alkaline Two-Dimensional Gel Electrophoresis -- Chromatin Immunoprecipitation of Replication Factors Moving with the Replication Fork -- Density Transfer as a Method to Analyze the Progression of DNA Replication Forks -- High-Resolution Mapping of Points of Site-Specific Replication Stalling -- DNA Replication in Nucleus-Free Xenopus Egg Extracts -- Genome-Wide Analysis Methods -- ChIP-Chip to Analyze the Binding of Replication Proteins to Chromatin Using Oligonucleotide DNA Microarrays -- Analyzing Origin Activation Patterns by Copy Number Change Experiments -- Detection of Replication Origins Using Comparative Genomics and Recombinational ARS Assay -- Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes -- Application of Alkaline Sucrose Gradient Centrifugation in the Analysis of DNA Replication After DNA Damage -- Biochemistry and Biophysics Methods -- Isolation of Recombinant DNA Elongation Proteins -- In Vitro Assays for Studying Helicase Activities -- The Use of 2-Aminopurine Fluorescence to Study DNA Polymerase Function -- Single-Molecule Observation of Prokaryotic DNA Replication -- Cell Biology and Genetic Methods -- Visualization of DNA Replication Sites in Mammalian Nuclei -- Cell-Cycle Synchrony for Analysis of S. pombe DNA Replication -- Measuring DNA Content by Flow Cytometry in Fission Yeast -- Microscopy Techniques to Examine DNA Replication in Fission Yeast -- Using the DHFR Heat-Inducible Degron for Protein Inactivation in Schizosaccharomyces pombe -- Assays Used to Study the DNA Replication Checkpoint in Fission Yeast -- Incorporation of Thymidine Analogs for Studying Replication Kinetics in Fission Yeast -- The Fast-Halo Assay for the Assessment of DNA Damage at the Single-Cell Level -- Monitoring Homologous Recombination Following Replication Fork Perturbation in the Fission Yeast Schizosaccharomyces pombe -- DNA Fiber Analysis Methods -- Computational Methods to Study Kinetics of DNA Replication -- Use of DNA Combing to Study DNA Replicationin Xenopus and Human Cell-Free Systems -- Electron Microscopy Methods for Studying In Vivo DNA Replication Intermediates -- Determining the Replication Dynamics of Specific Gene Loci by Single-Molecule Analysis of Replicated DNA -- Use of DNA Combing for Studying DNA Replication In Vivo in Yeast and Mammalian Cells.
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650 |
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|a Biochemistry.
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650 |
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|a Genetic engineering.
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650 |
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|a Animal genetics.
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650 |
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0 |
|a Biochemical engineering.
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650 |
1 |
4 |
|a Biochemistry, general.
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650 |
2 |
4 |
|a Genetic Engineering.
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650 |
2 |
4 |
|a Animal Genetics and Genomics.
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650 |
2 |
4 |
|a Biochemical Engineering.
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700 |
1 |
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|a Vengrova, Sonya.
|e editor.
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700 |
1 |
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|a Dalgaard, Jacob Z.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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773 |
0 |
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|t Springer eBooks
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