DNA Replication : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Vengrova, Sonya. (Editor ), Dalgaard, Jacob Z. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:1st ed. 2009.
Colección:Methods in Molecular Biology, 521
Materias:
Tabla de Contenidos:
  • Reviews
  • DNA Replication Initiation
  • DNA Replication Fork Proteins
  • Random and Site-Specific Replication Termination
  • Checkpoint Regulation of DNA Replication
  • to Molecular Combing: Genomics, DNA Replication, and Cancer
  • Methods for Analysis of Replication of a Single Locus
  • Replication Initiation Point Mapping: Approach and Implications
  • Purification of Restriction Fragments Containing Replication Intermediates from Complex Genomes for 2-D Gel Analysis
  • Topological Analysis of Plasmid DNA Replication Intermediates Using Two-Dimensional Agarose Gels
  • Analysis of Telomeric DNA Replication Using Neutral–Alkaline Two-Dimensional Gel Electrophoresis
  • Chromatin Immunoprecipitation of Replication Factors Moving with the Replication Fork
  • Density Transfer as a Method to Analyze the Progression of DNA Replication Forks
  • High-Resolution Mapping of Points of Site-Specific Replication Stalling
  • DNA Replication in Nucleus-Free Xenopus Egg Extracts
  • Genome-Wide Analysis Methods
  • ChIP-Chip to Analyze the Binding of Replication Proteins to Chromatin Using Oligonucleotide DNA Microarrays
  • Analyzing Origin Activation Patterns by Copy Number Change Experiments
  • Detection of Replication Origins Using Comparative Genomics and Recombinational ARS Assay
  • Isolation of Restriction Fragments Containing Origins of Replication from Complex Genomes
  • Application of Alkaline Sucrose Gradient Centrifugation in the Analysis of DNA Replication After DNA Damage
  • Biochemistry and Biophysics Methods
  • Isolation of Recombinant DNA Elongation Proteins
  • In Vitro Assays for Studying Helicase Activities
  • The Use of 2-Aminopurine Fluorescence to Study DNA Polymerase Function
  • Single-Molecule Observation of Prokaryotic DNA Replication
  • Cell Biology and Genetic Methods
  • Visualization of DNA Replication Sites in Mammalian Nuclei
  • Cell-Cycle Synchrony for Analysis of S. pombe DNA Replication
  • Measuring DNA Content by Flow Cytometry in Fission Yeast
  • Microscopy Techniques to Examine DNA Replication in Fission Yeast
  • Using the DHFR Heat-Inducible Degron for Protein Inactivation in Schizosaccharomyces pombe
  • Assays Used to Study the DNA Replication Checkpoint in Fission Yeast
  • Incorporation of Thymidine Analogs for Studying Replication Kinetics in Fission Yeast
  • The Fast-Halo Assay for the Assessment of DNA Damage at the Single-Cell Level
  • Monitoring Homologous Recombination Following Replication Fork Perturbation in the Fission Yeast Schizosaccharomyces pombe
  • DNA Fiber Analysis Methods
  • Computational Methods to Study Kinetics of DNA Replication
  • Use of DNA Combing to Study DNA Replicationin Xenopus and Human Cell-Free Systems
  • Electron Microscopy Methods for Studying In Vivo DNA Replication Intermediates
  • Determining the Replication Dynamics of Specific Gene Loci by Single-Molecule Analysis of Replicated DNA
  • Use of DNA Combing for Studying DNA Replication In Vivo in Yeast and Mammalian Cells.