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160520s2016 xxu| s |||| 0|eng d |
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|a 9781493936649
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| 024 |
7 |
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|a 10.1007/978-1-4939-3664-9
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a Gap Junction Protocols /
|c edited by Mathieu Vinken, Scott R. Johnstone.
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| 250 |
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|a 1st ed. 2016.
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| 260 |
# |
# |
|a New York, NY :
|b Springer New York :
|b Imprint: Humana,
|c 2016.
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| 300 |
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|a XIII, 235 p. 45 illus., 34 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1437
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| 505 |
0 |
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|a Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction -- DNA Methylation Analysis of Human Tissue-Specific Connexin Genes -- Detection of Connexins in Liver Cells Using Sodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis -- Immunohisto- and Cytochemistry Analysis of Connexins -- Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells -- Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor -- Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells -- Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis -- Scrape Loading/Dye Transfer Assay -- Microinjection Technique for Assessment of Gap Junction Function -- Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling -- Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro -- Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photo-Activation and Fluorescence Imaging -- A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication of Second Messengers in Cultured Bone Cells -- Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels -- Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for the Measurement of Gap Junction Conductance.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Vinken, Mathieu.
|e editor.
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| 700 |
1 |
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|a Johnstone, Scott R.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
|