Gap Junction Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Vinken, Mathieu. (Editor ), Johnstone, Scott R. (Editor )
Formato: eBook
Lenguaje:English
Publicado: New York, NY : Springer New York : Imprint: Humana, 2016.
Edición:1st ed. 2016.
Colección:Methods in Molecular Biology, 1437
Materias:
Tabla de Contenidos:
  • Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction
  • DNA Methylation Analysis of Human Tissue-Specific Connexin Genes
  • Detection of Connexins in Liver Cells Using Sodiumdodecylsulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis
  • Immunohisto- and Cytochemistry Analysis of Connexins
  • Small Interfering RNA-Mediated Connexin Gene Knockdown in Vascular Endothelial and Smooth Muscle Cells
  • Generation and Use of Trophoblast Stem Cells and Uterine Myocytes to Study the Role of Connexins for Pregnancy and Labor
  • Identification of Connexin43 Phosphorylation and S-Nitrosylation in Cultured Primary Vascular Cells
  • Preparation of Gap Junctions in Membrane Microdomains for Immunoprecipitation and Mass Spectrometry Interactome Analysis
  • Scrape Loading/Dye Transfer Assay
  • Microinjection Technique for Assessment of Gap Junction Function
  • Electroporation Loading and Dye Transfer: A Safe and Robust Method to Probe Gap Junctional Coupling
  • Using Fluorescence Recovery After Photobleaching to Study Gap Junctional Communication In Vitro
  • Tracking Dynamic Gap Junctional Coupling in Live Cells by Local Photo-Activation and Fluorescence Imaging
  • A Functional Assay to Assess Connexin43-Mediated Cell-to-Cell Communication of Second Messengers in Cultured Bone Cells
  • Calcium Wave Propagation Triggered by Local Mechanical Stimulation as a Method for Studying Gap Junctions and Hemichannels
  • Establishment of the Dual Whole Cell Recording Patch Clamp Configuration for the Measurement of Gap Junction Conductance.