Genomic Imprinting : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Ward, Andrew. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2002.
Edición:1st ed. 2002.
Colección:Methods in Molecular Biology, 181
Materias:
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020 |a 9781592592111 
024 7 |a 10.1385/1592592112  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a Genomic Imprinting :  |b Methods and Protocols /  |c edited by Andrew Ward. 
250 |a 1st ed. 2002. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2002. 
300 |a XII, 377 p. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
338 |a online resource  |b cr  |2 rdacarrier 
490 1 |a Methods in Molecular Biology,  |v 181 
505 0 |a Generation of Monoparental Embryos for Investigation into Genomic Imprinting -- Deriving and Propagating Mouse Embryonic Stem Cell Lines for Studying Genomic Imprinting -- Balanced Translocations for the Analysis of Imprinted Regions of the Mouse Genome -- Production of YAC Transgenic Mice by Pronuclear Injection -- A Transgenic Approach to Studying Imprinted Genes -- Methylation-Sensitive Genome Scanning -- Subtraction-Hybridization Method for the Identification of Imprinted Genes -- Identification of Imprinted Loci by Methylation -- Ribonuclease Protection -- Quantitative RT-PCR-Based Analysis of Allele-Specific Gene Expression -- Allele-Specific In Situ Hybridization (ASISH) -- RNA-FISH to Analyze Allele-Specific Expression -- Flow Cytometry and FISH to Investigate Allele-Specific Replication Timing and Homologous Association of Imprinted Chromosomes -- Southern Analysis Using Methyl-Sensitive Restriction Enzymes -- A PCR-Based Method for Studying DNA Methylation -- Bisulfite-Based Methylation Analysis of Imprinted Genes -- Direct Analysis of Chromosome Methylation -- In Vitro Methylation of Predetermined Regions in Recombinant DNA Constructs -- In Vitro Methylation of Specific Regions in Recombinant DNA Constructs by Excision and Religation -- Detection of Methyl?Sensitive DNA?Binding Proteins with Possible Involvement in the Imprinting Phenomenon -- Probing Chromatin Structure with Nuclease Sensitivity Assays -- Examining Histone Acetlylation at Specific Genomic Regions -- Purification of the MeCP2/Histone Deacetylase Complex from Xenopus laevis -- Reconstitution of Chromatin In Vitro -- Genomic Imprinting in Plants. 
650 0 |a Human genetics. 
650 1 4 |a Human Genetics. 
700 1 |a Ward, Andrew.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks