Genomic Imprinting : Methods and Protocols /
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| Format: | eBook |
| Language: | English |
| Published: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2002.
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| Edition: | 1st ed. 2002. |
| Series: | Methods in Molecular Biology,
181 |
| Subjects: |
Table of Contents:
- Generation of Monoparental Embryos for Investigation into Genomic Imprinting
- Deriving and Propagating Mouse Embryonic Stem Cell Lines for Studying Genomic Imprinting
- Balanced Translocations for the Analysis of Imprinted Regions of the Mouse Genome
- Production of YAC Transgenic Mice by Pronuclear Injection
- A Transgenic Approach to Studying Imprinted Genes
- Methylation-Sensitive Genome Scanning
- Subtraction-Hybridization Method for the Identification of Imprinted Genes
- Identification of Imprinted Loci by Methylation
- Ribonuclease Protection
- Quantitative RT-PCR-Based Analysis of Allele-Specific Gene Expression
- Allele-Specific In Situ Hybridization (ASISH)
- RNA-FISH to Analyze Allele-Specific Expression
- Flow Cytometry and FISH to Investigate Allele-Specific Replication Timing and Homologous Association of Imprinted Chromosomes
- Southern Analysis Using Methyl-Sensitive Restriction Enzymes
- A PCR-Based Method for Studying DNA Methylation
- Bisulfite-Based Methylation Analysis of Imprinted Genes
- Direct Analysis of Chromosome Methylation
- In Vitro Methylation of Predetermined Regions in Recombinant DNA Constructs
- In Vitro Methylation of Specific Regions in Recombinant DNA Constructs by Excision and Religation
- Detection of Methyl?Sensitive DNA?Binding Proteins with Possible Involvement in the Imprinting Phenomenon
- Probing Chromatin Structure with Nuclease Sensitivity Assays
- Examining Histone Acetlylation at Specific Genomic Regions
- Purification of the MeCP2/Histone Deacetylase Complex from Xenopus laevis
- Reconstitution of Chromatin In Vitro
- Genomic Imprinting in Plants.