Glycoprotein Methods and Protocols : The Mucins /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Corfield, Anthony P. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2000.
Edición:1st ed. 2000.
Colección:Methods in Molecular Biology, 125
Materias:
Tabla de Contenidos:
  • Purification of Mucin
  • Isolation of Large Gel-Forming Mucins
  • Preparation of Membrane Mucin
  • Detection and Ouantitation of Mucin
  • Histologically Based Methods for Detection of Mucin
  • Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods
  • The Gastrointestinal Adherent Mucous Gel Barrier
  • Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling
  • Separation, Identification, and Physical Characterization of Mucins
  • Separation and Identification of Mucins and Their Glycoforms
  • Heterogeneity and Size Distribution of Gel-Forming Mucins
  • Rheology of Mucin
  • Rheology of Mucin
  • Mucin Peptide Analysis
  • Amino Acid Analysis of Mucins
  • Identification of Glycosylation Sites in Mucin Peptides by Edman Degradation
  • Synthetic Peptides for the Analysis and Preparation of Antimucin Antibodies
  • Mucin Domains to Explore Disulfide-Dependent Dimer Formation
  • Carbohydrate Structural Analysis of Mucins
  • Monosaccharide Composition of Mucins
  • O-Linked Oligosaccharide Chain Release and Fractionation
  • Structural Analysis of Mucin-Type O-Linked Oligosaccharides
  • Measurement of Sulfate in Mucins
  • Mucin Biosynthesis
  • Biosynthesis of Mucin Cell and Organ Culture Methods for Biosynthetic Study
  • Metabolic Labeling Methods for the Preparation and Biosynthetic Study of Mucin
  • Identification of Mucins Using Metabolic Labeling, Immunoprecipitation, and Gel Electrophoresis
  • Mucin Precursors Identification and Analysis of Their Intracellular Processing
  • Inhibition of Mucin Glycosylation
  • O-Linked Chain Glycosyltransferases
  • Mucin Gene Detection
  • Mucin cDNA Cloning
  • Northern Blot Analysis of Large mRNAs
  • Southern Blot Analysis of Large DNA Fragments
  • In Situ Hybridization Techniques for Localizing Mucin mRNA
  • Detection of Mucin Gene Polymorphism
  • Preparation of Antimucin Antibodies
  • Polyclonal and Monoclonal Techniques
  • Monoclonal Antibodies to Mucin VNTR Peptides
  • Enzymatic Degradation of Mucins
  • Mucinase Activity
  • Proteinase Activity
  • Glycosidase Activity
  • Assays for Bacterial Mucin-Desulfating Sulfatases
  • Mucin-Bacterial Interactions
  • Mucin-Bacterial Binding Assays
  • Growth of Mucin Degrading Bacteria in Biofilms
  • Cellular And Humoral Responses To Mucins
  • Generation of MUC1 CytotoxicT-Cells in Mice and Epitope Mapping
  • Analysis of the Frequency of MHC-Unrestricted MUC1 -Specific CytotoxicT-Cells in Peripheral Blood by Limiting Dilution Assay
  • Expression of MUC1 in Insect Cells Using Recombinant Baculovirus
  • Mucin Antigen Presentation Using Dendritic Cells
  • Detection of Humoral Immune Responses to Mucins.