Glycoprotein Methods and Protocols : The Mucins /
Autor Corporativo: | |
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Otros Autores: | |
Formato: | eBook |
Lenguaje: | English |
Publicado: |
Totowa, NJ :
Humana Press : Imprint: Humana,
2000.
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Edición: | 1st ed. 2000. |
Colección: | Methods in Molecular Biology,
125 |
Materias: |
Tabla de Contenidos:
- Purification of Mucin
- Isolation of Large Gel-Forming Mucins
- Preparation of Membrane Mucin
- Detection and Ouantitation of Mucin
- Histologically Based Methods for Detection of Mucin
- Detection and Quantitation of Mucins Using Chemical, Lectin, and Antibody Methods
- The Gastrointestinal Adherent Mucous Gel Barrier
- Quantitation of Biosynthesis and Secretion of Mucin Using Metabolic Labeling
- Separation, Identification, and Physical Characterization of Mucins
- Separation and Identification of Mucins and Their Glycoforms
- Heterogeneity and Size Distribution of Gel-Forming Mucins
- Rheology of Mucin
- Rheology of Mucin
- Mucin Peptide Analysis
- Amino Acid Analysis of Mucins
- Identification of Glycosylation Sites in Mucin Peptides by Edman Degradation
- Synthetic Peptides for the Analysis and Preparation of Antimucin Antibodies
- Mucin Domains to Explore Disulfide-Dependent Dimer Formation
- Carbohydrate Structural Analysis of Mucins
- Monosaccharide Composition of Mucins
- O-Linked Oligosaccharide Chain Release and Fractionation
- Structural Analysis of Mucin-Type O-Linked Oligosaccharides
- Measurement of Sulfate in Mucins
- Mucin Biosynthesis
- Biosynthesis of Mucin Cell and Organ Culture Methods for Biosynthetic Study
- Metabolic Labeling Methods for the Preparation and Biosynthetic Study of Mucin
- Identification of Mucins Using Metabolic Labeling, Immunoprecipitation, and Gel Electrophoresis
- Mucin Precursors Identification and Analysis of Their Intracellular Processing
- Inhibition of Mucin Glycosylation
- O-Linked Chain Glycosyltransferases
- Mucin Gene Detection
- Mucin cDNA Cloning
- Northern Blot Analysis of Large mRNAs
- Southern Blot Analysis of Large DNA Fragments
- In Situ Hybridization Techniques for Localizing Mucin mRNA
- Detection of Mucin Gene Polymorphism
- Preparation of Antimucin Antibodies
- Polyclonal and Monoclonal Techniques
- Monoclonal Antibodies to Mucin VNTR Peptides
- Enzymatic Degradation of Mucins
- Mucinase Activity
- Proteinase Activity
- Glycosidase Activity
- Assays for Bacterial Mucin-Desulfating Sulfatases
- Mucin-Bacterial Interactions
- Mucin-Bacterial Binding Assays
- Growth of Mucin Degrading Bacteria in Biofilms
- Cellular And Humoral Responses To Mucins
- Generation of MUC1 CytotoxicT-Cells in Mice and Epitope Mapping
- Analysis of the Frequency of MHC-Unrestricted MUC1 -Specific CytotoxicT-Cells in Peripheral Blood by Limiting Dilution Assay
- Expression of MUC1 in Insect Cells Using Recombinant Baculovirus
- Mucin Antigen Presentation Using Dendritic Cells
- Detection of Humoral Immune Responses to Mucins.