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140106s2014 xxu| s |||| 0|eng d |
| 020 |
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|a 9781627037648
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| 024 |
7 |
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|a 10.1007/978-1-62703-764-8
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
0 |
|a DNA Cloning and Assembly Methods /
|c edited by Svein Valla, Rahmi Lale.
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| 250 |
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|a 1st ed. 2014.
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| 260 |
# |
# |
|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2014.
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| 300 |
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|a XI, 303 p. 74 illus., 50 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 1116
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| 505 |
0 |
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|a BioBrick Assembly Standards and Techniques and Associated Software Tools -- Plasmid Construction by SLIC or Sequence and Ligation Independent Cloning -- Quick and Clean Cloning -- Hierarchical Ligation-Independent Assembly of PCR Fragments -- USER-Derived Cloning Methods and Their Primer Design -- Application of the Restriction-Free (RF) Cloning for Multi-Components Assembly -- A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform -- Circular Polymerase Extension Cloning -- Golden Gate Cloning -- Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy -- FX cloning: A Simple and Robust High-Throughput Cloning Method for Protein Expression -- Minimum GC-rich Sequences for Overlap Extension PCR and Primer Annealing -- Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR -- Combinatorial Assembly of Clone Libraries Using Site-Specific Recombination -- Application of In-Fusion™ Cloning for the Parallel Construction of Escherichia coli Expression Vectors -- SeamLess Ligation Cloning Extract（SLiCE）Cloning Method -- j5 DNA Assembly Design Automation -- FASTPCR Software for PCR, in silico PCR, and Oligonucleotide Assembly and Analysis.
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| 650 |
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0 |
|a Human genetics.
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| 650 |
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0 |
|a Medical genetics.
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| 650 |
1 |
4 |
|a Human Genetics.
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| 650 |
2 |
4 |
|a Gene Function.
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| 700 |
1 |
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|a Valla, Svein.
|e editor.
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| 700 |
1 |
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|a Lale, Rahmi.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
|
| 773 |
0 |
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|t Springer eBooks
|