DNA Cloning and Assembly Methods /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Valla, Svein. (Editor ), Lale, Rahmi. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2014.
Edición:1st ed. 2014.
Colección:Methods in Molecular Biology, 1116
Materias:
Tabla de Contenidos:
  • BioBrick Assembly Standards and Techniques and Associated Software Tools
  • Plasmid Construction by SLIC or Sequence and Ligation Independent Cloning
  • Quick and Clean Cloning
  • Hierarchical Ligation-Independent Assembly of PCR Fragments
  • USER-Derived Cloning Methods and Their Primer Design
  • Application of the Restriction-Free (RF) Cloning for Multi-Components Assembly
  • A Single-Tube Assembly of DNA Using the Transfer-PCR (TPCR) Platform
  • Circular Polymerase Extension Cloning
  • Golden Gate Cloning
  • Design and Construction of Multigenic Constructs for Plant Biotechnology Using the GoldenBraid Cloning Strategy
  • FX cloning: A Simple and Robust High-Throughput Cloning Method for Protein Expression
  • Minimum GC-rich Sequences for Overlap Extension PCR and Primer Annealing
  • Simple Cloning and DNA Assembly in Escherichia coli by Prolonged Overlap Extension PCR
  • Combinatorial Assembly of Clone Libraries Using Site-Specific Recombination
  • Application of In-Fusion™ Cloning for the Parallel Construction of Escherichia coli Expression Vectors
  • SeamLess Ligation Cloning Extract(SLiCE)Cloning Method
  • j5 DNA Assembly Design Automation
  • FASTPCR Software for PCR, in silico PCR, and Oligonucleotide Assembly and Analysis.