Epidermal Cells : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Turksen, Kursad. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2010.
Edición:2nd ed. 2010.
Colección:Methods in Molecular Biology, 585
Materias:
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245 1 0 |a Epidermal Cells :  |b Methods and Protocols /  |c edited by Kursad Turksen. 
250 |a 2nd ed. 2010. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2010. 
300 |a XVI, 446 p. 85 illus., 29 illus. in color. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
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490 1 |a Methods in Molecular Biology,  |v 585 
505 0 |a Isolation of Adult Mouse Stem Keratinocytes Using Magnetic Cell Sorting (MACS) -- Functional Investigations of Keratinocyte Stem Cells and Progenitors at a Single-Cell Level Using Multiparallel Clonal Microcultures -- Growth and Stratification of Epithelial Cells in Minimal Culture Conditions -- Matched Cultures of Keratinocytes and Fibroblasts Derived from Normal and NER-Deficient Mouse Models -- Establishment of Spontaneously Immortalized Keratinocyte Lines from Wild-Type and Mutant Mice -- Study of Epidermal Differentiation in Human Keratinocytes Cultured in Autocrine Conditions -- Directed Differentiation of Human Embryonic Stem Cells to Epidermal Progenitors -- Expression and Analysis of Exogenous Proteins in Epidermal Cells -- Using siRNA Knockdown in HaCaT Cells to Study Transcriptional Control of Epidermal Proliferation Potential -- RNA Interference in Keratinocytes and an Organotypic Model of Human Epidermis -- Scanning for Transcription Factor Binding by a Variant EMSA -- Chromatin Immunoprecipitation for Identifying Transcription Factor Targets in Keratinocytes -- Gene Expression Profiling of Mouse Epidermal Keratinocytes -- Analysis of Tissue-Specific Gene Expression Using Laser Capture Microdissection -- Comprehensive Transcriptional Profiling of Human Epidermis, Reconstituted Epidermal Equivalents, and Cultured Keratinocytes Using DNA Microarray Chips -- Molecular Profiling of the Epidermis: A Proteomics Approach -- Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridization -- Embryonic Mammary Anlagen Analysis Using Immunolabelling of Whole Mounts -- Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis -- Tetracycline-Regulated Gene Expression in Transgenic Mouse Epidermis -- A Versatile Murine 3D Organotypic Model to Evaluate Aspects of Wound Healing and Epidermal Organization -- Optical and Biochemical Dissection of Connexin and Disease-Linked Connexin Mutants in 3D Organotypic Epidermis -- Cytokine Release in Tissue-Engineered Epidermal Equivalents After Prolonged Mechanical Loading -- Three-Dimensional Human Tissue Models of Wounded Skin -- In Vivo Transplantation of Genetically Modified Mouse Embryonic Epidermis -- A Transplant Model for Human Epidermal Skin Regeneration -- Identification of Epithelial Stem Cells In Vivo and In Vitro Using Keratin 19 and BrdU -- Isolation and Culture of Hair Follicle Pluripotent Stem (hfPS) Cells and Their Use for Nerve and Spinal Cord Regeneration -- Limiting Dilution Analysis of Murine Epidermal Stem Cells Using an In Vivo Regeneration Assay. 
650 0 |a Cell biology. 
650 0 |a Cell culture. 
650 1 4 |a Cell Biology. 
650 2 4 |a Cell Culture. 
700 1 |a Turksen, Kursad.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks 
900 |a Libro descargado a ALEPH en bloque (proveniente de proveedor)