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100715s2010 xxu| s |||| 0|eng d |
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|a 9781607613800
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024 |
7 |
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|a 10.1007/978-1-60761-380-0
|2 doi
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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245 |
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|a Epidermal Cells :
|b Methods and Protocols /
|c edited by Kursad Turksen.
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250 |
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|a 2nd ed. 2010.
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260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2010.
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300 |
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|a XVI, 446 p. 85 illus., 29 illus. in color. :
|b online resource.
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336 |
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|a text
|b txt
|2 rdacontent
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|a computer
|b c
|2 rdamedia
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|a online resource
|b cr
|2 rdacarrier
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|a Methods in Molecular Biology,
|v 585
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|a Isolation of Adult Mouse Stem Keratinocytes Using Magnetic Cell Sorting (MACS) -- Functional Investigations of Keratinocyte Stem Cells and Progenitors at a Single-Cell Level Using Multiparallel Clonal Microcultures -- Growth and Stratification of Epithelial Cells in Minimal Culture Conditions -- Matched Cultures of Keratinocytes and Fibroblasts Derived from Normal and NER-Deficient Mouse Models -- Establishment of Spontaneously Immortalized Keratinocyte Lines from Wild-Type and Mutant Mice -- Study of Epidermal Differentiation in Human Keratinocytes Cultured in Autocrine Conditions -- Directed Differentiation of Human Embryonic Stem Cells to Epidermal Progenitors -- Expression and Analysis of Exogenous Proteins in Epidermal Cells -- Using siRNA Knockdown in HaCaT Cells to Study Transcriptional Control of Epidermal Proliferation Potential -- RNA Interference in Keratinocytes and an Organotypic Model of Human Epidermis -- Scanning for Transcription Factor Binding by a Variant EMSA -- Chromatin Immunoprecipitation for Identifying Transcription Factor Targets in Keratinocytes -- Gene Expression Profiling of Mouse Epidermal Keratinocytes -- Analysis of Tissue-Specific Gene Expression Using Laser Capture Microdissection -- Comprehensive Transcriptional Profiling of Human Epidermis, Reconstituted Epidermal Equivalents, and Cultured Keratinocytes Using DNA Microarray Chips -- Molecular Profiling of the Epidermis: A Proteomics Approach -- Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridization -- Embryonic Mammary Anlagen Analysis Using Immunolabelling of Whole Mounts -- Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis -- Tetracycline-Regulated Gene Expression in Transgenic Mouse Epidermis -- A Versatile Murine 3D Organotypic Model to Evaluate Aspects of Wound Healing and Epidermal Organization -- Optical and Biochemical Dissection of Connexin and Disease-Linked Connexin Mutants in 3D Organotypic Epidermis -- Cytokine Release in Tissue-Engineered Epidermal Equivalents After Prolonged Mechanical Loading -- Three-Dimensional Human Tissue Models of Wounded Skin -- In Vivo Transplantation of Genetically Modified Mouse Embryonic Epidermis -- A Transplant Model for Human Epidermal Skin Regeneration -- Identification of Epithelial Stem Cells In Vivo and In Vitro Using Keratin 19 and BrdU -- Isolation and Culture of Hair Follicle Pluripotent Stem (hfPS) Cells and Their Use for Nerve and Spinal Cord Regeneration -- Limiting Dilution Analysis of Murine Epidermal Stem Cells Using an In Vivo Regeneration Assay.
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650 |
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|a Cell biology.
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650 |
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|a Cell culture.
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650 |
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|a Cell Biology.
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650 |
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4 |
|a Cell Culture.
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700 |
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|a Turksen, Kursad.
|e editor.
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710 |
2 |
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|a SpringerLink (Online service)
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773 |
0 |
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|t Springer eBooks
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900 |
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|a Libro descargado a ALEPH en bloque (proveniente de proveedor)
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