Epidermal Cells : Methods and Protocols /

Bibliographic Details
Corporate Author: SpringerLink (Online service)
Other Authors: Turksen, Kursad. (Editor)
Format: eBook
Language:English
Published: Totowa, NJ : Humana Press : Imprint: Humana, 2010.
Edition:2nd ed. 2010.
Series:Methods in Molecular Biology, 585
Subjects:
Table of Contents:
  • Isolation of Adult Mouse Stem Keratinocytes Using Magnetic Cell Sorting (MACS)
  • Functional Investigations of Keratinocyte Stem Cells and Progenitors at a Single-Cell Level Using Multiparallel Clonal Microcultures
  • Growth and Stratification of Epithelial Cells in Minimal Culture Conditions
  • Matched Cultures of Keratinocytes and Fibroblasts Derived from Normal and NER-Deficient Mouse Models
  • Establishment of Spontaneously Immortalized Keratinocyte Lines from Wild-Type and Mutant Mice
  • Study of Epidermal Differentiation in Human Keratinocytes Cultured in Autocrine Conditions
  • Directed Differentiation of Human Embryonic Stem Cells to Epidermal Progenitors
  • Expression and Analysis of Exogenous Proteins in Epidermal Cells
  • Using siRNA Knockdown in HaCaT Cells to Study Transcriptional Control of Epidermal Proliferation Potential
  • RNA Interference in Keratinocytes and an Organotypic Model of Human Epidermis
  • Scanning for Transcription Factor Binding by a Variant EMSA
  • Chromatin Immunoprecipitation for Identifying Transcription Factor Targets in Keratinocytes
  • Gene Expression Profiling of Mouse Epidermal Keratinocytes
  • Analysis of Tissue-Specific Gene Expression Using Laser Capture Microdissection
  • Comprehensive Transcriptional Profiling of Human Epidermis, Reconstituted Epidermal Equivalents, and Cultured Keratinocytes Using DNA Microarray Chips
  • Molecular Profiling of the Epidermis: A Proteomics Approach
  • Detection of Gene Expression in Embryonic Tissues and Stratified Epidermis by In Situ Hybridization
  • Embryonic Mammary Anlagen Analysis Using Immunolabelling of Whole Mounts
  • Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis
  • Tetracycline-Regulated Gene Expression in Transgenic Mouse Epidermis
  • A Versatile Murine 3D Organotypic Model to Evaluate Aspects of Wound Healing and Epidermal Organization
  • Optical and Biochemical Dissection of Connexin and Disease-Linked Connexin Mutants in 3D Organotypic Epidermis
  • Cytokine Release in Tissue-Engineered Epidermal Equivalents After Prolonged Mechanical Loading
  • Three-Dimensional Human Tissue Models of Wounded Skin
  • In Vivo Transplantation of Genetically Modified Mouse Embryonic Epidermis
  • A Transplant Model for Human Epidermal Skin Regeneration
  • Identification of Epithelial Stem Cells In Vivo and In Vitro Using Keratin 19 and BrdU
  • Isolation and Culture of Hair Follicle Pluripotent Stem (hfPS) Cells and Their Use for Nerve and Spinal Cord Regeneration
  • Limiting Dilution Analysis of Murine Epidermal Stem Cells Using an In Vivo Regeneration Assay.