Epidermal Cells : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Turksen, Kursad. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2005.
Edición:1st ed. 2005.
Colección:Methods in Molecular Biology, 289
Materias:
Tabla de Contenidos:
  • Keratinocyte and Organ Cultures
  • Primary Mouse Keratinocyte Culture
  • Serial Cultivation of Primary Adult Murine Keratinocytes
  • Keratinocyte Culture in the Absence of Substrate Attachment
  • Application of Genetically Modified Feeder Cells for Culture of Keratinocytes
  • Organ Culture of Developing Mouse Skin and Its Application for Molecular Mechanistic Studies of Morphogenesis
  • Experimental Models to Analyze Differentiation Functions of Cultured Keratinocytes In Vitro and In Vivo
  • In Vitro Fabrication of Engineered Human Skin
  • Epidermal Stem Cells
  • In Vivo Labeling and Analysis of Epidermal Stem Cells
  • Method for the Harvest and Assay of In Vitro Clonogenic Keratinocytes Stem Cells From Mice
  • FACS Enrichment of Human Keratinocyte Stem Cells
  • Isolation, Characterization, and Culture of Epithelial Stem Cells
  • Keratin 19 as a Stem Cell Marker In Vivo and In Vitro
  • Analysis of Epidermal Differentiation
  • Immunolocalization in the Epidermis
  • Epidermal Cell Analysis by RT-PCR
  • Whole-Mount Assays for Gene Induction and Barrier Formation in the Developing Epidermis
  • Analysis of Early Epidermal Development in Zebrafish
  • Analysis of E2F Factors During Epidermal Differentiation
  • Analysis of HOX Homeodomain Proteins and Gene Transcripts in the Epidermis
  • Apoptosis in the Epidermis
  • Fate of Desmosomal Proteins in Apoptotic Epidermal Cells
  • Analysis of Connexin 43 Expression on Keratinocytes Using Flow Cytometry
  • MMP-9 and TIMP-1 Assays in Keratinocyte Cultures
  • Characterization of Epithelial Cells in the Hair Follicle With S100 Proteins
  • Immunoelectron Microscopic Analysis of Cornified Cell Envelopes and Antigen Retrieval
  • Methods and Approaches for the Analysis for Epidermal Function
  • Cell Kinetic Analysis in Artificial Skin Using Immunochemical Methods
  • Proliferation, Differentiation, and Inflammation in Normal and Hyperproliferative Skin Using Multiparameter Flow Cytometry
  • Fluorimetric DNA Assay of Cell Number
  • Keratinocyte Transient Transfections
  • Tetracycline-Regulated Gene Expression in Epidermal Keratinocytes
  • Gene Targeting by Oligonucleotides in Keratinocytes
  • Promoter Analysis in the Human SPRR Gene Family
  • Stable Integration of Large PAC Constructs in Keratinocytes
  • Targeted Somatic Mutagenesis in the Mouse Epidermis
  • Methods to Study Protein-Protein Interactions
  • Isolation of Recombinant Phage-Displayed Antibodies Recognizing Skin Keratinocytes
  • Analysis of Tissue-Specific DNA Methylation During Development
  • Serial Analysis of Gene Expression in Human Keratinocytes and Epidermis
  • Methods for Gene Expression Profiling in Dermatology Research Using DermArray® Nylon Filter DNA Microarrays
  • Two-Photon Fluorescence Imaging and Reactive Oxygen Species Detection Within the Epidermis
  • Transplantation and Gene Therapy
  • In Vivo Transplantation of Engineered Human Skin
  • Epidermis-Targeted Gene Transfer Using In Vivo Electroporation
  • Gene and Stem Cell Therapy of the Hair Follicle.