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100301s2000 xxu| s |||| 0|eng d |
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|a 9781592590636
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| 024 |
7 |
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|a 10.1385/1592590632
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Extracellular Matrix Protocols /
|c edited by Charles Streuli, Michael Grant.
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| 250 |
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|a 1st ed. 2000.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2000.
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| 300 |
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|a XIV, 370 p. 50 illus. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 139
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| 505 |
0 |
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|a Biochemistry of Extracellular Matrix -- Semipermeabilized Cells to Study Procollagen Assembly -- Biophysics of Extracellular Matrix Proteins -- Quantitative Determination of Collagen Crosslinks -- Analysis of Laminin Structure and Function with Recombinant Glycoprotein Expressed in Insect Cells -- Recombinant Collagen Trimers from Insect Cells and Yeast -- Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag -- Preparation of Isotopically Labeled Recombinant Fragments of Fibronectin for Functional and Structural Study by Heteronuclear Nuclear Magnetic Resonance Spectroscopy -- Reconstitution of Functional Integrin into Phopsholipid Vesicles and Planar Lipid Bilayers -- Confocal-FRAP Analysis of ECM Molecular Interactions -- Electron Cryomicroscopy of Fibrillar Collagens -- Atomic Force Microspopy Measurements of Intermolecular Binding Strength -- ECM Macromolecules: Rotary Shadowing and Scanning Transmission Electron Microscopy -- Molecular Biology of Extracellular Matrix Genes -- Screening for Mutations in Cartilage ECM Genes -- Tissue-Specific KO of ECM Proteins -- Homologous Gene Targeting to Study ECM Assembly -- Enhancer Analysis of the ?1(II) and ?2(XI) Collagen Genes in Transfected Chondrocytes and Transgenic Mice -- Retroviral Delivery of ECM Genes to Cells -- Cell Biology of the Extracellular Matrix -- Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Subtrates -- Methods for Preparing Extracellular Matrix and Quantifying Insulin-like Growth Factor-Binding Protein Binding to the ECM -- Measuring Interactions Between ECM and TGF?-Like Proteins -- Using Organotypic Tissue Slices as Substrata for the Culture of Dissociated Cells -- Neuroepithelial Differentiation Induced by ECM Molecules -- Migration Assays for Oligodendrocyte Precursor Cells -- Cell Adhesion Assays -- Tissue Engineering and Cell-Populated Collagen Matrices -- Solid Phase Assays for Studying ECM Protein-Protein Interactions -- Tissue Engineering of Cartilage -- Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes -- Fluorescence Assays to Study Cell Adhesion and Migration In Vitro -- Analyzing Cell-ECM Interactions in Adult Mammary Gland by Transplantation of Embryonic Mammary Tissue from Knockout Mice.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
1 |
4 |
|a Cell Biology.
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| 700 |
1 |
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|a Streuli, Charles.
|e editor.
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| 700 |
1 |
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|a Grant, Michael.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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