Extracellular Matrix Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Streuli, Charles. (Editor ), Grant, Michael. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2000.
Edición:1st ed. 2000.
Colección:Methods in Molecular Biology, 139
Materias:
Tabla de Contenidos:
  • Biochemistry of Extracellular Matrix
  • Semipermeabilized Cells to Study Procollagen Assembly
  • Biophysics of Extracellular Matrix Proteins
  • Quantitative Determination of Collagen Crosslinks
  • Analysis of Laminin Structure and Function with Recombinant Glycoprotein Expressed in Insect Cells
  • Recombinant Collagen Trimers from Insect Cells and Yeast
  • Eukaryotic Expression and Purification of Recombinant Extracellular Matrix Proteins Carrying the Strep II Tag
  • Preparation of Isotopically Labeled Recombinant Fragments of Fibronectin for Functional and Structural Study by Heteronuclear Nuclear Magnetic Resonance Spectroscopy
  • Reconstitution of Functional Integrin into Phopsholipid Vesicles and Planar Lipid Bilayers
  • Confocal-FRAP Analysis of ECM Molecular Interactions
  • Electron Cryomicroscopy of Fibrillar Collagens
  • Atomic Force Microspopy Measurements of Intermolecular Binding Strength
  • ECM Macromolecules: Rotary Shadowing and Scanning Transmission Electron Microscopy
  • Molecular Biology of Extracellular Matrix Genes
  • Screening for Mutations in Cartilage ECM Genes
  • Tissue-Specific KO of ECM Proteins
  • Homologous Gene Targeting to Study ECM Assembly
  • Enhancer Analysis of the ?1(II) and ?2(XI) Collagen Genes in Transfected Chondrocytes and Transgenic Mice
  • Retroviral Delivery of ECM Genes to Cells
  • Cell Biology of the Extracellular Matrix
  • Using Self-Assembled Monolayers to Pattern ECM Proteins and Cells on Subtrates
  • Methods for Preparing Extracellular Matrix and Quantifying Insulin-like Growth Factor-Binding Protein Binding to the ECM
  • Measuring Interactions Between ECM and TGF?-Like Proteins
  • Using Organotypic Tissue Slices as Substrata for the Culture of Dissociated Cells
  • Neuroepithelial Differentiation Induced by ECM Molecules
  • Migration Assays for Oligodendrocyte Precursor Cells
  • Cell Adhesion Assays
  • Tissue Engineering and Cell-Populated Collagen Matrices
  • Solid Phase Assays for Studying ECM Protein-Protein Interactions
  • Tissue Engineering of Cartilage
  • Tissue Recombinants to Study Extracellular Matrix Targeting to Basement Membranes
  • Fluorescence Assays to Study Cell Adhesion and Migration In Vitro
  • Analyzing Cell-ECM Interactions in Adult Mammary Gland by Transplantation of Embryonic Mammary Tissue from Knockout Mice.