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100301s2009 xxu| s |||| 0|eng d |
| 020 |
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|a 9781597454094
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| 024 |
7 |
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|a 10.1007/978-1-59745-409-4
|2 doi
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| 040 |
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|a Sistema de Bibliotecas del Tecnológico de Costa Rica
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| 245 |
1 |
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|a Genetic Modification of Hematopoietic Stem Cells :
|b Methods and Protocols /
|c edited by Christopher Baum.
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| 250 |
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|a 1st ed. 2009.
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| 260 |
# |
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|a Totowa, NJ :
|b Humana Press :
|b Imprint: Humana,
|c 2009.
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| 300 |
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|a XXII, 490 p. 84 illus., 10 illus. in color. :
|b online resource.
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| 336 |
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|a text
|b txt
|2 rdacontent
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| 337 |
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|a computer
|b c
|2 rdamedia
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| 338 |
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|a online resource
|b cr
|2 rdacarrier
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| 490 |
1 |
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|a Methods in Molecular Biology,
|v 506
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| 505 |
0 |
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|a Immunomagnetic Enrichment of Human and Mouse Hematopoietic Stem Cells for Gene Therapy Applications -- Isolation of Human and Mouse Hematopoietic Stem Cells -- Murine Hematopoietic Stem Cell Transduction Using Retroviral Vectors -- Genetic Modification of Human Hematopoietic Cells: Preclinical Optimization of Oncoretroviral-mediated Gene Transfer for Clinical Trials -- Short-Term Culture of Human CD34+ Cells for Lentiviral Gene Transfer -- T Cell Culture for Gammaretroviral Transfer -- Retroviral Transduction of Murine Primary T Lymphocytes -- Lentiviral Vector Gene Transfer into Human T Cells -- DNA Transposons for Modification of Human Primary T Lymphocytes -- Retroviral Gene Transfer into Primary Human Natural Killer Cells -- Lentiviral Vector-Mediated Genetic Programming of Mouse and Human Dendritic Cells -- In Situ (In Vivo) Gene Transfer into Murine Bone Marrow Stem Cells -- In Vivo and Ex Vivo Gene Transfer in Thymocytes and Thymocyte Precursors -- Design and Production of Retro- and Lentiviral Vectors for Gene Expression in Hematopoietic Cells -- Knock-Down of Gene Expression in Hematopoietic Cells -- The Use of Retroviral Vectors for tet-Regulated Gene Expression in Cell Populations -- Detection of Replication Competent Retrovirus and Lentivirus -- Release Testing of Retroviral Vectors and Gene-Modified Cells -- Copy Number Determination of Genetically-Modified Hematopoietic Stem Cells -- Tissue Procurement for Molecular Studies Using Laser-Assisted Microdissection -- Leukemia Diagnosis in Murine Bone Marrow Transplantation Models -- Humanized Mouse Models to Study the Human Haematopoietic Stem Cell Compartment -- Canine Models of Gene-Modified Hematopoiesis -- Detection of Retroviral Integration Sites by Linear Amplification-Mediated PCR and Tracking of Individual Integration Clones in Different Samples -- Retroviral Insertion Site Analysis in Dominant Haematopoietic Clones -- Tracking Gene-Modified T Cells In Vivo -- DNA Microarray Studies of Hematopoietic Subpopulations -- Quantification of Genomic Mutations in Murine Hematopoietic Cells -- Proteomics Studies After Hematopoietic Stem Cell Transplantation -- Spectral Karyotyping and Fluorescence In Situ Hybridization of Murine Cells -- Database Setup for Preclinical Studies of Gene-Modified Hematopoiesis -- The US and EU Regulatory Perspectives on the Clinical Use of Hematopoietic Stem/Progenitor Cells Genetically Modified Ex Vivo by Retroviral Vectors.
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| 650 |
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0 |
|a Biochemistry.
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| 650 |
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0 |
|a Cell biology.
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| 650 |
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0 |
|a Stem cells.
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| 650 |
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0 |
|a Gene therapy.
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| 650 |
1 |
4 |
|a Biochemistry, general.
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| 650 |
2 |
4 |
|a Cell Biology.
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| 650 |
2 |
4 |
|a Stem Cells.
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| 650 |
2 |
4 |
|a Gene Therapy.
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| 700 |
1 |
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|a Baum, Christopher.
|e editor.
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| 710 |
2 |
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|a SpringerLink (Online service)
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| 773 |
0 |
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|t Springer eBooks
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