DNA Methylation : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Tost, Jörg. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:2nd ed. 2009.
Colección:Methods in Molecular Biology, 507
Materias:
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020 |a 9781597455220 
024 7 |a 10.1007/978-1-59745-522-0  |2 doi 
040 |a Sistema de Bibliotecas del Tecnológico de Costa Rica 
245 1 0 |a DNA Methylation :  |b Methods and Protocols /  |c edited by Jörg Tost. 
250 |a 2nd ed. 2009. 
260 # # |a Totowa, NJ :  |b Humana Press :  |b Imprint: Humana,  |c 2009. 
300 |a XVI, 392 p. :  |b online resource. 
336 |a text  |b txt  |2 rdacontent 
337 |a computer  |b c  |2 rdamedia 
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490 1 |a Methods in Molecular Biology,  |v 507 
505 0 |a DNA Methylation: An Introduction to the Biology and the Disease-Associated Changes of a Promising Biomarker -- Global Methylation Levels -- Quantification of Global DNA Methylation by Capillary Electrophoresis and Mass Spectrometry -- Methyl Group Acceptance Assay for the Determination of Global DNA Methylation Levels -- Methods For Whole Genome Analysis Of Dna Methylation Patterns -- Immunodetection Array -- Methylated DNA Immunoprecipitation (MeDIP) -- The MIRA Method for DNA Methylation Analysis -- The Help Assay -- Differential Methylation Hybridization: Profiling DNA Methylation with a High-Density CpG Island Microarray -- Analysis of DNA Methylation by Amplification of Intermethylated Sites (AIMS) -- Methylation-Sensitive Representational Difference Analysis (MS-RDA) -- Restriction Landmark Genomic Scanning: Analysis of CpG Islands in Genomes by 2D Gel Electrophoresis -- GoldenGate® Assay for DNA Methylation Profiling -- 5?-Azacytidine Expression Arrays -- Gene-Specific Methylation Analysis -- DNA Methylation Analysis by Bisulfite Conversion, Cloning, and Sequencing of Individual Clones -- Identification and Quantification of Differentially Methylated Loci by the Pyrosequencing™ Technology -- Mass Spectrometric Analysis of Cytosine Methylation by Base-Specific Cleavage and Primer Extension Methods -- Melting Curve Assays for DNA Methylation Analysis -- Methylation SNaPshot: A Method for the Quantification of Site-Specific DNA Methylation Levels -- Bio-COBRA: Absolute Quantification of DNA Methylation in Electrofluidics Chips -- Restriction Digestion and Real-Time PCR (qAMP) -- MethylQuant: A Real-Time PCR-Based Method to Quantify DNA Methylation at Single Specific Cytosines -- Methylation-Specific PCR -- MethyLight -- Quantification of Methylated DNA by HeavyMethyl Duplex PCR -- Special Applications -- Analysis of Methylated Circulating DNA in Cancer Patients’ Blood -- Prevention of PCR Cross-Contamination by UNG Treatment of Bisulfite-Treated DNA -- Profiling DNA Methylation from Small Amounts of Genomic DNA Starting Material: Efficient Sodium Bisulfite Conversion and Subsequent Whole-Genome Amplification. 
650 0 |a Human genetics. 
650 0 |a Gene expression. 
650 1 4 |a Human Genetics. 
650 2 4 |a Gene Expression. 
700 1 |a Tost, Jörg.  |e editor. 
710 2 |a SpringerLink (Online service) 
773 0 |t Springer eBooks