DNA Methylation : Methods and Protocols /

Detalles Bibliográficos
Autor Corporativo: SpringerLink (Online service)
Otros Autores: Tost, Jörg. (Editor )
Formato: eBook
Lenguaje:English
Publicado: Totowa, NJ : Humana Press : Imprint: Humana, 2009.
Edición:2nd ed. 2009.
Colección:Methods in Molecular Biology, 507
Materias:
Tabla de Contenidos:
  • DNA Methylation: An Introduction to the Biology and the Disease-Associated Changes of a Promising Biomarker
  • Global Methylation Levels
  • Quantification of Global DNA Methylation by Capillary Electrophoresis and Mass Spectrometry
  • Methyl Group Acceptance Assay for the Determination of Global DNA Methylation Levels
  • Methods For Whole Genome Analysis Of Dna Methylation Patterns
  • Immunodetection Array
  • Methylated DNA Immunoprecipitation (MeDIP)
  • The MIRA Method for DNA Methylation Analysis
  • The Help Assay
  • Differential Methylation Hybridization: Profiling DNA Methylation with a High-Density CpG Island Microarray
  • Analysis of DNA Methylation by Amplification of Intermethylated Sites (AIMS)
  • Methylation-Sensitive Representational Difference Analysis (MS-RDA)
  • Restriction Landmark Genomic Scanning: Analysis of CpG Islands in Genomes by 2D Gel Electrophoresis
  • GoldenGate® Assay for DNA Methylation Profiling
  • 5?-Azacytidine Expression Arrays
  • Gene-Specific Methylation Analysis
  • DNA Methylation Analysis by Bisulfite Conversion, Cloning, and Sequencing of Individual Clones
  • Identification and Quantification of Differentially Methylated Loci by the Pyrosequencing™ Technology
  • Mass Spectrometric Analysis of Cytosine Methylation by Base-Specific Cleavage and Primer Extension Methods
  • Melting Curve Assays for DNA Methylation Analysis
  • Methylation SNaPshot: A Method for the Quantification of Site-Specific DNA Methylation Levels
  • Bio-COBRA: Absolute Quantification of DNA Methylation in Electrofluidics Chips
  • Restriction Digestion and Real-Time PCR (qAMP)
  • MethylQuant: A Real-Time PCR-Based Method to Quantify DNA Methylation at Single Specific Cytosines
  • Methylation-Specific PCR
  • MethyLight
  • Quantification of Methylated DNA by HeavyMethyl Duplex PCR
  • Special Applications
  • Analysis of Methylated Circulating DNA in Cancer Patients’ Blood
  • Prevention of PCR Cross-Contamination by UNG Treatment of Bisulfite-Treated DNA
  • Profiling DNA Methylation from Small Amounts of Genomic DNA Starting Material: Efficient Sodium Bisulfite Conversion and Subsequent Whole-Genome Amplification.